Herein, a high‐performance photoelectrochemical (PEC) bioassay system based on the PEC and oxidase‐catalytic coupled reactions at an air–liquid–solid triphase joint interface is reported. The triphase joint interface is constructed by immobilizing oxidases on the top of hydrophobic TiO₂ nanowire arrays grown on transparent fluorine‐doped tin oxide substrates. Such triphase reaction systems allow sufficient oxygen to constantly and rapidly transport to the reaction zone from the air phase directly, which greatly enhances and stabilizes the oxidase‐catalytic reaction, leading to a ≈100‐fold wider linear detection range and a higher detection accuracy compared with conventional liquid–solid diphase systems. Moreover, the single crystal nanowire arrays, possessing rapid photogenerated charge transport abilities, give the triphase PEC bioassay system a 32 times lower detection limit and a 12.8 times higher sensitivity compared with that of a randomly packed nanoparticle‐based system. The design principle proposed here can be generalized to develop high‐performance PEC bioassay systems for accurate analyte determination, given the availability, for a variety of oxidases.

中文翻译：

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基于TiO2纳米线阵列的三相界面的有效生物光电化学反应

在此，报告了一种基于PEC和气-液-固三相接头界面上的氧化酶催化偶联反应的高性能光电化学（PEC）生物测定系统。通过将氧化酶固定在疏水性TiO ₂的顶部来构建三相接头界面在透明的掺氟氧化锡衬底上生长的纳米线阵列。此类三相反应系统可提供足够的氧气，以直接将其从空气中连续快速地直接输送到反应区，从而大大增强和稳定了氧化酶催化反应，从而使线性检测范围扩大了约100倍，检测精度更高与传统的液固两相系统。此外，单晶纳米线阵列具有快速的光生电荷传输能力，与基于随机包装的纳米粒子系统相比，三相PEC生物测定系统的检测限低32倍，灵敏度高12.8倍。可以将此处提出的设计原理推广到开发高性能PEC生物测定系统，以进行准确的分析物测定，