In the face of looming threats from multi-drug resistant microorganisms, there is a growing need for technologies that will enable rapid identification and drug susceptibility profiling of these pathogens in health care settings. In particular, recent progress in microfluidics and nucleic acid amplification is pushing the boundaries of timescale for diagnosing bacterial infections. With a diverse range of techniques and parallel developments in the field of analytical chemistry, an integrative perspective is needed to understand the significance of these developments. This review examines the scope of new developments in assay technologies grouped by key enabling domains of research. First, we examine recent development in nucleic acid amplification assays for rapid identification and drug susceptibility testing in bacterial infections. Next, we examine advances in microfluidics that facilitate acceleration of diagnostic assays via integration and scale. Lastly, recentdevelopments in biosensor technologies are reviewed. We conclude this review with perspectives on the use of emerging concepts to develop paradigm-changing assays.

中文翻译：

---

快速的病原体鉴定和药敏试验的新兴分析技术。

面对来自多重耐药微生物的迫在眉睫的威胁，对能够在医疗机构中对这些病原体进行快速识别和药物敏感性分析的技术的需求日益增长。特别是，微流控技术和核酸扩增的最新进展推动了诊断细菌感染的时间表的界限。随着分析化学领域的各种技术和并行发展，需要一个综合的视角来理解这些发展的重要性。这篇综述按主要的研究领域对检测技术的新发展范围进行了研究。首先，我们检查了核酸扩增测定法在细菌感染中快速鉴定和药敏试验的最新进展。下一个，我们研究了微流体技术的进展，这些进展通过集成和规模化促进了诊断分析的加速。最后，回顾了生物传感器技术的最新发展。我们以对新兴概念的使用来发展范式改变分析的观点来结束本综述。