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Local cell coordination does not alter individual cell migration during collective migration but does impact cellular exchange events.
Integrative Biology ( IF 2.5 ) Pub Date : 2019-04-01 , DOI: 10.1093/intbio/zyz015
Benjamin Slater 1 , Edwin Ng 1 , Alison P McGuigan 1, 2
Affiliation  

Coordinated cell re-organization is critical to ensure correct tissue morphogenesis for a number of important embryonic and tissue repair events, however the mechanisms that govern cells coordination during collective movements, particularly in situations where cells are spatially restricted by their neighbours, are not well understood. Here we assessed cell re-organization in monolayers of retinal epithelial cells (ARPE-19) to determine if cells that coordinate with their neighbours exhibit differential migration properties to non-coordinating cells and participate differently in local cell re-organization of the tissue sheet. From global tracking analysis, we determined that the movement profiles of cells were indistinguishable regardless of whether or not they were a part of multicellular streams. Using high magnification live imaging of cell membranes, we also characterized the localized geometry and organization of a monolayer (cell area, number of nearest neighbours, aspect ratio, internal cell angles) during cell re-organization in both streaming and non-streaming regions. Consistent with our global migration analysis, we observed no differences in cell sheet geometry and organization in streaming versus non-streaming regions. We did however observe that cells executed T1-like transitions to exchange position within the space-limited monolayer and that exchange events consistently involved at least one non-streaming cell. Our data suggests a model in which cell movement within the sheet is limited by neighbour exchange events and likely cells transition between streaming and non-streaming regimes to facilitate these neighbour exchange events while maintaining the integrity of the sheet.

中文翻译:

局部细胞协调在集体迁移过程中不会改变单个细胞的迁移,但会影响细胞交换事件。

协调一致的细胞重组对于确保许多重要的胚胎和组织修复事件的正确组织形态形成至关重要,但是,人们对在集体运动过程中控制细胞协调的机制(尤其是在细胞受到邻居空间限制的情况下)了解得很少。在这里,我们评估了视网膜上皮细胞(ARPE-19)单层中的细胞重组,以确定与其邻居协调的细胞是否表现出向非协调细胞的差异迁移特性,并以不同的方式参与组织表层的局部细胞重组。通过全局跟踪分析,我们确定细胞的运动曲线是无法区分的,无论它们是否是多细胞流的一部分。使用细胞膜的高倍率实时成像,我们还表征了在流和非流区域中的细胞重组过程中单层的局部几何形状和组织(细胞面积,最近邻的数量,纵横比,内部细胞角度)。与我们的全球迁移分析一致,我们观察到流域和非流域中细胞表的几何形状和组织没有差异。但是,我们确实观察到,细胞执行T1样跃迁以交换空间受限单层内的位置,并且交换事件始终涉及至少一个非流式细胞。
更新日期:2019-05-31
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