BACKGROUND The potassium dichromate oxidation method used in determination of alcohols in fermentation has two major disadvantages. This method cannot be used to determine alcohols in raw fermentation broth samples, which often contain various reducing sugars. The method is not environment friendly due to the carcinogenicity of Cr (VI) used. RESULTS A new method for determination of reducing sugars and total alcohols in raw fermentation broths was developed. The fermentation broth was pretreated to remove proteins, polysaccharides, glycerol and organic acids. The colorimetric change from both total alcohols and reducing sugars by potassium permanganate oxidation was measured. The portion of colorimetric change from oxidation of reducing sugars was determined by DNS test and subtracted. The remaining portion of colorimetric change was then used to calculate the total alcohol concentration in the sample. CONCLUSIONS Using this method, total alcohol concentration can be easily and accurately determined in both distilled samples and raw fermentation broth samples. It is fast and environmental friendly.

中文翻译：

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一种用于测定发酵液中总酒精含量的高通量方法。

背景技术用于测定发酵中的醇的重铬酸钾氧化方法具有两个主要缺点。该方法不能用于测定原始发酵液样品中的醇，这些样品通常含有各种还原糖。由于所用的Cr（VI）具有致癌性，因此该方法对环境不利。结果建立了测定生发酵液中还原糖和总醇含量的新方法。对发酵液进行预处理以去除蛋白质，多糖，甘油和有机酸。测量了由高锰酸钾氧化引起的总醇和还原糖的比色变化。通过DNS测试确定还原糖氧化产生的比色变化部分，并将其减去。然后将比色变化的其余部分用于计算样品中的总酒精浓度。结论使用该方法，可以轻松，准确地确定蒸馏样品和生发酵液样品中的总酒精浓度。它既快速又环保。