It has been reported that MicroRNAs (miRNAs) play pivotal roles in the occurrence and progression of a variety of cancers. As reported, miR-4295 promotes cell growth and metastasis in a lot of cancers. Nonetheless, the role and molecular mechanism of miR-4295 in HNSCC still remain unknown. In this study, we discovered miR-4295 expression was significantly upregulated in HNSCC tissues and cell lines, which is also associated with the overall survival of patients. Additionally, suppression of miR-4295 significantly inhibited cell proliferation, migration and EMT process in HNSCC. Through Targetscan website, it was predicted that NPTX1 might be a direct target gene of miR-4295. Then, we verified that NPTX1 could directly interact with miR-4295 via luciferase reporter and RNA assays. What's more, we discovered that there was a significantly negative correlation between NPTX1 and miR-4295 expression. It was indicated by further investigation that the effect of miR-4295 suppression on cell proliferation, migration and EMT process in HNSCC can be restored by knockdown of NPTX1 at the same time. Our results suggested that miR-4295 promoted the progression of HNSCC via regulating NPTX1 expression and miR-4295/NPTX1 axis, which may be a new therapeutic strategy for HNSCC.

中文翻译：

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MiR-4295通过靶向NPTX1促进头颈部鳞状细胞癌中的细胞增殖和转移。

据报道，MicroRNA（miRNA）在多种癌症的发生和发展中起关键作用。据报道，miR-4295促进许多癌症中的细胞生长和转移。尽管如此，miR-4295在HNSCC中的作用和分子机制仍然未知。在这项研究中，我们发现miR-4295在HNSCC组织和细胞系中的表达明显上调，这也与患者的整体存活率有关。此外，miR-4295的抑制显着抑制了HNSCC中的细胞增殖，迁移和EMT过程。通过Targetscan网站，预测NPTX1可能是miR-4295的直接靶基因。然后，我们验证了NPTX1可以通过萤光素酶报告基因和RNA分析直接与miR-4295相互作用。更重要的是，我们发现NPTX1和miR-4295表达之间存在显着的负相关。进一步的研究表明，miR-4295抑制对HNSCC细胞增殖，迁移和EMT过程的影响可以通过同时敲低NPTX1来恢复。我们的结果表明，miR-4295通过调节NPTX1表达和miR-4295 / NPTX1轴促进了HNSCC的发展，这可能是HNSCC的一种新的治疗策略。