A Eu^III-containing single molecule BCR-Eu as design platform for ratio-metric fluorescent sensor which includes a blue-emitting coumarin dye, a green-emitting BODIPY fluorophore and a Eu^III moiety as the origin of red light has been designed and synthesized. The compound BCR shows only green emission with large stoke shift when excited in 400 nm due to good fluorescence resonance energy transfer from coumarin to BODIPY. After embedding Eu^III complexes in the molecule, BCR-Eu exhibits dual emission which is equal in magnitude and independent of each other, when excited at the range of 305–365 nm. An emission from Lanthanide complexes as the stable built-in standard fluorescence peak offers a promising opportunity to enhance the precision of bioimaging and also an ideal design platform for future ratio-metric fluorescent sensor.

已设计并合成了含有Eu ^III的单分子BCR-Eu作为比率量度荧光传感器的设计平台，该传感器包括发出蓝色的香豆素染料，发出绿色的BODIPY荧光团和Eu ^III部分作为红光的来源。 。由于良好的荧光共振能量从香豆素到BODIPY的转移，化合物BCR在400 nm激发时仅显示具有大斯托克位移的绿色发射。在分子中嵌入Eu ^III络合物后，BCR-Eu在305–365 nm范围内激发时，呈现出大小相等且彼此独立的双重发射。镧系元素配合物发出的光作为稳定的内置标准荧光峰，为提高生物成像的精度提供了一个有前途的机会，同时也是将来比率式荧光传感器的理想设计平台。