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Tasselseed5 encodes a cytochrome C oxidase that functions in sex determination by affecting jasmonate catabolism in maize.
Journal of Integrative Plant Biology ( IF 11.4 ) Pub Date : 2019-11-01 , DOI: 10.1111/jipb.12826
Fei Wang 1 , Zhenjiang Yuan 1, 2, 3 , Zhiwei Zhao 1, 2 , Caixia Li 4 , Xin Zhang 1 , Huafeng Liang 1, 2 , Yawen Liu 1 , Qian Xu 4 , Hongtao Liu 1
Affiliation  

Maize (Zea mays L.) is a monoecious grass plant in which mature male and female florets form the tassel and ear, respectively. Maize is often used as a model plant to study flower development. Several maize tassel seed mutants, such as the recessive mutants tasselseed1 (ts1) and tasselseed2 (ts2), exhibit a reversal in sex determination, which leads to the generation of seeds in tassels. The phenotype of the dominant mutant, Tasselseed5 (Ts5), is similar to that of ts2. Here, we positionally cloned the underlying gene of Ts5 and characterized its function. We show that the GRMZM2G177668 gene is overexpressed in Ts5. This gene encodes a cytochrome C oxidase, which catalyzes the transformation of jasmonoyl‐L‐isoleucine (JA‐Ile) to 12OH‐JA‐Ile during jasmonic acid catabolism. Consistent with this finding, no JA‐Ile peak was detected in Ts5 tassels during the sex determination period, unlike in the wild type. Transgenic maize plants overexpressing GRMZM2G177668 exhibited a tassel‐seed phenotype similar to that of Ts5. These results indicate that the JA‐Ile peak in tassels is critical for sex determination and that the Ts5 mutant phenotype results from the disruption of this peak in tassels during sex determination.

中文翻译:

Tasselseed5编码一种细胞色素C氧化酶,其通过影响玉米的茉莉酸酯分解代谢而在性别决定中起作用。

玉米(Zea mays L.)是一种雌雄同株的草植物,成熟的雄性和雌性小花分别形成流苏和穗。玉米通常用作研究花卉发育的典范植物。几个玉米流苏种子突变体,例如隐性突变体tasselseed1ts1)和tasselseed2ts2),在性别决定方面表现出逆转,从而导致流苏中种子的产生。显性突变体Tasselseed5Ts5)的表型与ts2的表型相似。在这里,我们在位置上克隆了Ts5的基础基因并表征其功能。我们显示GRMZM2G177668基因在Ts5中过表达。此基因编码一种细胞色素C氧化酶,在茉莉酸分解代谢过程中,它催化茉莉单酰-L-异亮氨酸(JA-Ile)向12OH-JA-Ile的转化。与该发现一致的是,与野生型不同,在性别确定时期的Ts5 t中未检测到JA-Ile峰。过度表达GRMZM2G177668的转基因玉米植株表现出与Ts5相似的流苏种子表型。这些结果表明,t子中的JA-Ile峰对于性别决定至关重要,而Ts5突变表型是由性别决定时t子中该峰值的破坏产生的。
更新日期:2019-11-01
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