Covering: up to 2019As abundant natural products, isoprenoids have many useful industrial applications in the manufacturing of drugs, fragrances, food additives, colorants, rubber and advanced biofuels. The microbial production of isoprenoids has received much attention in recent years. Metabolic engineering approaches and synthetic biology have been utilized to reconstruct and optimize the metabolic pathways for isoprenoid production in cell factories. In this review, the recent advances in isoprenoid production using microbes are summarized, with a focus on MEP and MVA pathway engineering, downstream isoprenoid pathway engineering and microbial host engineering, which mainly includes central carbon pathway engineering. Finally, future perspectives for the improvement of isoprenoid production are discussed.

中文翻译：

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使用细胞工厂生产天然类异戊二烯的代谢工程学策略的最新进展。

涵盖范围：截止到2019年，类异戊二烯作为丰富的天然产物，在药物，香料，食品添加剂，着色剂，橡胶和高级生物燃料的制造中具有许多有用的工业应用。近年来，类异戊二烯的微生物生产受到了广泛关注。代谢工程方法和合成生物学已被用于重建和优化细胞工厂中类异戊二烯生产的代谢途径。在这篇综述中，总结了使用微生物生产类异戊二烯的最新进展，重点是MEP和MVA途径工程，下游类异戊二烯途径工程和微生物宿主工程，其中主要包括中央碳途径工程。最后，讨论了改善类异戊二烯生产的未来前景。