The contamination of pesticide residues in Oolong tea has raised much concern in recent years. The objective of this study was to synthesize gold nanoparticles (AuNPs) and develop surface-enhanced Raman spectroscopy (SERS) methods for detection and quantification of pesticides in Oolong tea. Facile synthesis of spherical and monodispersed AuNPs with an average diameter of 15 nm was achieved, which induced strong electromagnetic fields in SERS analysis. AuNP substrates were employed for rapid detection and quantification of carbendazim in Tieguanyin Oolong tea. Partial least squares (PLS) analysis and leave-one-out cross validation were utilized in spectral data analysis. The PLS results for Oolong tea samples were obtained: R value = 0.964; the detection limit = 100 μg/kg. These results demonstrate that SERS coupled with gold nanoparticle substrate is a simple, rapid, and sensitive analytical tool for measurement and quantification of carbendazim residues in Oolong tea.

近年来，乌龙茶中农药残留的污染引起了人们的广泛关注。这项研究的目的是合成金纳米颗粒（AuNPs）并开发用于检测和定量乌龙茶中农药的表面增强拉曼光谱（SERS）方法。实现了平均直径为15 nm的球形和单分散AuNP的轻松合成，这在SERS分析中引起了强电磁场。AuNP底物用于铁观音乌龙茶中多菌灵的快速检测和定量。光谱数据分析中使用了偏最小二乘（PLS）分析和留一法交叉验证。获得了乌龙茶样品的PLS结果：R值= 0.964; 检出限= 100μg/ kg。这些结果表明，SERS结合金纳米颗粒基质是一种用于测量和定量乌龙茶中多菌灵残留的简单，快速，灵敏的分析工具。