Nanoparticles camouflaged by red blood cell (RBC) membranes have attracted considerable attention owing to reservation of structure of membrane and surface proteins, endowing prominent cell-specific function including biocompatibility, prolonged circulation lifetime, and reduced reticular endothelial system (RES) uptake ability. Considering the drawbacks of carrier-free nanomedicine including the serious drug burst release, poor stability, and lack of immune escape function, herein we developed and fabricated a novel RBC membranes biomimetic combinational therapeutic system by enveloping the small molecular drug coassemblies of 10-hydroxycamptothecin (10-HCPT) and indocyanine green (ICG) in the RBC membranes for prolonged circulation, controlled drug release, and synergistic chemo-photothermal therapy (PTT). The self-reorganized [email protected] nanoparticles (NPs) exhibited a diameter of ∼150 nm with core–shell structure, high drug payload (∼92 wt %), and reduced RES uptake function. Taking advantage of the stealth functionality of RBC membranes, [email protected] NPs remarkably enhanced the accumulation at the tumor sites by passive targeting followed by cellular endocytosis. Upon the stimuli of near-infrared laser followed by acidic stimulation, [email protected] NPs showed exceptional instability by heat-mediated membrane disruption and pH change, thereby triggering the rapid disassembly and accelerated drug release. Consequently, compared with individual treatment, [email protected] NPs under dual-stimuli accomplished highly efficient apoptosis in cancer cells and remarkable ablation of tumors by chemo-PTT. This biomimetic nanoplatform based on carrier-free, small molecular drug coassemblies integrating imaging capacity as a promising theranostic system provides potential for cancer diagnosis and combinational therapy.

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光/ pH触发的仿生红细胞膜伪装成小分子药物组合物，用于成像引导的组合化学光热疗法

由于保留了膜和表面蛋白质的结构，赋予细胞显着的特定功能，包括生物相容性，延长的循环寿命和降低的网状内皮系统（RES）吸收能力，被红细胞（RBC）膜伪装的纳米颗粒引起了广泛的关注。考虑到无载体纳米药物的缺点包括严重的药物爆发释放，稳定性差和缺乏免疫逃逸功能，本文我们通过包封10-羟基喜树碱的小分子药物组合体来开发和制造了新型的RBC膜仿生组合治疗系统。 10-HCPT）和RBC膜中的吲哚菁绿（ICG）用于延长循环，控制药物释放和协同化学光热疗法（PTT）。自重组[受电子邮件保护的]纳米颗粒（NPs）的直径约为150 nm，具有核壳结构，较高的药物有效负载量（〜92 wt％），并且RES吸收功能降低。利用RBC膜的隐身功能，[电子邮件保护] NP通过被动靶向和细胞内吞作用显着增强了在肿瘤部位的蓄积。在受到近红外激光刺激后再进行酸性刺激后，[受电子邮件保护的] NP通过热介导的膜破坏和pH值变化表现出异常的不稳定性，从而触发了快速分解和加速药物释放。因此，与单独治疗相比，在双重刺激下[受电子邮件保护的] NP在癌细胞中实现了高效的细胞凋亡，并通过化学PTT显着消融了肿瘤。