Advanced glycation end-products (AGEs) from non-enzymatic glycation are implicated in several degenerative diseases, including being a crucial contributor in secondary complications of diabetes. This has garnered significant scientific interest in inhibiting agents of AGEs to prevent and remediate disorders arising due to glycation. In the current study, inhibitory effects on AGEs formation were investigated using bio-enzymatically synthesized nanoformulations of gold (AuNPs) and silver (AgNPs) by physiologically important enzyme, β galactosidase. Human serum albumin, most abundant protein in human blood plasma, was glycated by incubating with glucose leading to AGEs formation. The AGEs formation was significantly minimized with both AuNPs and AgNPs, as confirmed by various biophysical and biochemical techniques. Circular dichroism and Fourier transform infrared spectroscopy further affirmed antiglycation potential of AuNPs and AgNPs. The results were corroborated with thiol group, free lysine and carbonyl content estimation for native, glycated and nanoparticles (NPs) treated samples. Confocal microscopic imaging was performed to exhibit glycation inhibiting potential of the NPs. The inhibition of AGEs was observed to be slightly stronger in case of AgNPs than AuNPs with both exhibiting promising results as potential anti-glycating agents. The study sheds light on potential of non-toxic NPs being utilized as controlling agents against hyperglycemic conditions and diabetes management.

中文翻译：

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通过生物酶法合成的金和银纳米制剂抑制人血清白蛋白的先进糖基化加合物：抵消高血糖状况的潜在工具。

非酶糖基化的高级糖基化终产物（AGEs）与多种退行性疾病有关，包括在糖尿病继发性并发症中起关键作用。在预防和补救由于糖基化引起的疾病的AGEs抑制剂中，这已经引起了广泛的科学兴趣。在当前的研究中，使用生理重要的酶β半乳糖苷酶通过生物酶法合成的金（AuNPs）和银（AgNPs）纳米制剂对AGEs形成的抑制作用进行了研究。人血浆白蛋白是人血浆中最丰富的蛋白质，可通过与葡萄糖孵育糖化糖化，从而形成AGEs。正如各种生物物理和生化技术所证实的那样，AuNPs和AgNPs显着最小化了AGEs形成。圆二色性和傅立叶变换红外光谱进一步证实了AuNPs和AgNPs的抗糖化潜力。天然，糖化和纳米颗粒（NPs）处理的样品的硫醇基团，游离赖氨酸和羰基含量估算结果得到了证实。进行共聚焦显微镜成像以显示NPs的糖基化抑制潜能。在AgNPs的情况下，观察到AGEs的抑制作用比AuNPs的抑制作用稍强，两者均显示出作为潜在的抗糖化剂的有希望的结果。该研究揭示了无毒NP被用作针对高血糖状况和糖尿病管理的控制剂的潜力。天然，糖化和纳米颗粒（NPs）处理样品的游离赖氨酸和羰基含量估算。进行共聚焦显微镜成像以显示NPs的糖基化抑制潜能。在AgNPs的情况下，观察到AGEs的抑制作用比AuNPs的抑制作用稍强，两者均显示出作为潜在的抗糖化剂的有希望的结果。该研究揭示了无毒NP被用作针对高血糖状况和糖尿病管理的控制剂的潜力。天然，糖化和纳米颗粒（NPs）处理样品的游离赖氨酸和羰基含量估算。进行共聚焦显微镜成像以显示NPs的糖基化抑制潜能。在AgNPs的情况下，观察到AGEs的抑制作用比AuNPs的抑制作用稍强，两者作为潜在的抗糖化剂均显示出有希望的结果。该研究揭示了无毒NP被用作针对高血糖症和糖尿病管理的控制剂的潜力。