Benzo [a]pyrene (BaP) is a model compound for the study of polycyclic aromatic hydrocarbon (PAH) carcinogenesis. Upon metabolism, BaP is metabolized to the ultimate metabolite, BaP trans-7,8-diol-anti-9,10-epoxide (BPDE), that reacts with cellular DNA to form BPDE-dG adducts responsible for BaP-induced mutagenicity, carcinogenicity, and teratogenicity. In this study, we employed our developed LC-MS/MS method to detect and quantity BPDE-dG adducts present in 42 normal human umbilical cord blood samples and 42 birth defect cases. We determined that there is no significant difference in the level of BPDE-dG formation between the normal and birth defect groups. This represents the first time to use an LC-MS/MS method to quantify BPDE-dG in human umbilical blood samples. The results indicated that under experimental conditions, BPDE-dG adducts were detected in all the human umbilical cord blood samples from the normal and birth defect groups.

中文翻译：

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通过 LC-MS/MS 分析检测人脐带血中的 BPDE-DNA 加合物

苯并 [a] 芘 (BaP) 是研究多环芳烃 (PAH) 致癌作用的模型化合物。代谢后，BaP 被代谢为最终代谢物 BaP trans-7,8-diol-anti-9,10-epoxide (BPDE)，它与细胞 DNA 反应形成 BPDE-dG 加合物，负责 BaP 诱导的致突变性、致癌性, 和致畸性。在这项研究中，我们采用我们开发的 LC-MS/MS 方法来检测和定量 42 例正常人脐带血样本和 42 例出生缺陷病例中存在的 BPDE-dG 加合物。我们确定正常组和出生缺陷组之间的 BPDE-dG 形成水平没有显着差异。这是首次使用 LC-MS/MS 方法对人脐血样本中的 BPDE-dG 进行量化。结果表明，在实验条件下，