Phenylenediamines (PDs), which are reported to cause allergic dermatitis and possess genotoxicity and carcinogenicity, are the ingredients used in permanent hair dyes. The fluorescent derivatization strategy coupled with micellar electrokinetic chromatography (MEKC) were established to analyze four PDs, including o-phenylenediamine (OPD), m-phenylenediamine (MPD), p-phenylenediamine (PPD) and toluene-2,5-diamine (PTD). Additionally, 5-(4, 6-dichlorotriazinyl) aminofluorescein (DTAF) was used as a fluorescent reagent derived at amino groups of PDs and underwent nucleophilic substitution reaction to improve the detection sensitivity. The derivatization condition reacted at 90 °C for 10 min in alkaline conditions. The optimized separation conditions were 20 mM borate (pH 8.0) containing 10 mM Brij 35 and 35% (v/v) methanol. The limits of detection (S/N = 3) for MPD, PTD, PPD and OPD were 25, 25, 50 and 100 nM, respectively. Compared to MEKC-UV, the sensitivity enhancements were 30- to 81-fold when PDs were derived with DTAF. The high-sensitivity MEKC-LIF method was successfully established and applied to determine PDs in commercial hair colors for quality control and in real hair samples for evaluating the location of PDs in dyed hair samples, as well as in percutaneous absorption samples for evaluating the ability of PDs to penetrate skin.

中文翻译：

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胶束电动色谱法测定 5-(4, 6-二氯三嗪基)氨基荧光素衍生的染发剂中苯二胺

据报道，苯二胺 (PD) 会引起过敏性皮炎并具有遗传毒性和致癌性，是永久性染发剂中使用的成分。建立荧光衍生化策略结合胶束电动色谱（MEKC）分析四种PD，包括邻苯二胺（OPD）、间苯二胺（MPD）、对苯二胺（PPD）和甲苯-2,5-二胺（PTD） ）。此外，5-（4, 6-二氯三嗪基）氨基荧光素（DTAF）用作衍生于PDs氨基的荧光试剂，并进行亲核取代反应以提高检测灵敏度。衍生条件在碱性条件下在 90 °C 下反应 10 分钟。优化的分离条件是含有 10 mM Brij 35 和 35% (v/v) 甲醇的 20 mM 硼酸盐 (pH 8.0)。MPD、PTD、PPD 和 OPD 的检测限 (S/N = 3) 分别为 25、25、50 和 100 nM。与 MEKC-UV 相比，当使用 DTAF 导出 PD 时，灵敏度提高了 30 到 81 倍。成功建立了高灵敏度 MEKC-LIF 方法，并将其应用于商业染发剂中用于质量控制的 PDs 测定、用于评估染发样品中 PDs 位置的真发样品中以及用于评估经皮吸收样品中的 PDs 能力的方法PDs 渗透皮肤。