Crop breeding aims to generate pure inbred lines with multiple desired traits. Doubled haploid (DH) and genome editing using CRISPR/Cas9 are two powerful game-changing technologies in crop breeding. However, both of them still fall short for rapid generation of pure elite lines with integrated favorable traits. Here, we report the development of a Haploid-Inducer Mediated Genome Editing (IMGE) approach, which utilizes a maize haploid inducer line carrying a CRISPR/Cas9 cassette targeting for a desired agronomic trait to pollinate an elite maize inbred line and to generate genome-edited haploids in the elite maize background. Homozygous pure DH lines with the desired trait improvement could be generated within two generations, thus bypassing the lengthy procedure of repeated crossing and backcrossing used in conventional breeding for integrating a desirable trait into elite commercial backgrounds.

作物育种旨在产生具有多个所需性状的纯近交系。使用CRISPR / Cas9的双倍单倍体（DH）和基因组编辑是作物育种中的两项强大的改变游戏规则的技术。然而，它们两者仍不足以快速产生具有综合有利特征的纯净品系。在这里，我们报告了单倍体诱导剂介导的基因组编辑（IMGE）方法的发展，该方法利用携带CRISPR / Cas9盒的玉米单倍体诱导物系针对所需的农艺性状授粉优良玉米近交系并产生基因组-在精英玉米背景中编辑了单倍体。具有所需性状改良的纯合纯DH系可以在两代内产生，