The development of genetic engineering in the 1970s marked a new frontier in genome-editing technology. Gene-editing technologies have provided a plethora of benefits to the life sciences. The clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/ Cas9) system is a versatile technology that provides the ability to add or remove DNA in the genome in a sequence-specific manner. Serious efforts are underway to improve the efficiency of CRISPR/Cas9 targeting and thus reduce off-target effects. Currently, various applications of CRISPR/Cas9 are used in cancer biology and oncology to perform robust site-specific gene editing, thereby becoming more useful for biological and clinical applications. Many variants and applications of CRISPR/Cas9 are being rapidly developed. Experimental approaches that are based on CRISPR technology have created a very promising tool that is inexpensive and simple for developing effective cancer therapeutics. This review discusses diverse applications of CRISPR-based gene-editing tools in oncology and potential future cancer therapies.

1970年代基因工程的发展标志着基因组编辑技术的新领域。基因编辑技术为生命科学提供了许多好处。所述Ç上光ř egularly我nterspaced小号园艺p alindromic řepeats / CRISPR相关蛋白9（CRISPR / Cas9）系统是一项通用技术，可提供以序列特异性方式在基因组中添加或去除DNA的功能。正在认真努力以提高CRISPR / Cas9靶向的效率，从而减少脱靶效应。目前，CRISPR / Cas9的各种应用已用于癌症生物学和肿瘤学中，以执行强大的位点特异性基因编辑，从而在生物学和临床应用中变得更加有用。CRISPR / Cas9的许多变体和应用正在快速开发中。基于CRISPR技术的实验方法创造了一种非常有前途的工具，该工具便宜且简单，可以开发出有效的癌症治疗剂。