Described in humans in 1894, Cryptococcus neoformans, a medically-important basidiomycete fungus, has since been studied in order to identify the factors that enable it to cause disease. Large-scale collections of mutants have been created, and mutant strains deposited in biorepositories. Studying these collections provides deeper functional insights for genes controlling biological processes, and ultimately complements a wealth of genomics and transcriptomics information. Therefore, to many laboratories use of these resources is profoundly insightful to study the molecular basis of cryptococcal pathobiology. The available collections of C. neoformans mutants also aid the molecular target identification and drug discovery efforts. To facilitate access to information, large-scale gene discoveries made from screening C. neoformans collections for over the past decade and a half are hereby compiled in a single document, and their key findings presented in brief. Cryptococcus neoformans is potentially the only basidiomycete yeast with extensively analyzed collections of mutants, and this makes it a plausible model for the creation of important collections for other basidiomycete fungi. Indeed, the genome-wide collections discussed in this present review represent a large sum of genes with associated phenotypes in virulence of this species on which prospective screens can rely as a reference point. Therefore, it is desired that studies underway will draw from these genome-wide screens to identify management strategies against fungal diseases.

自从1894年被描述为人类以来，就对新隐球菌（一种医学上重要的担子菌真菌）进行了研究，以确定导致其致病的因素。已经创建了大规模的突变体集合，并将突变体菌株保存在生物存储库中。对这些集合的研究为控制生物过程的基因提供了更深入的功能见解，并最终补充了丰富的基因组学和转录组学信息。因此，对于许多实验室而言，利用这些资源来研究隐球菌病理生物学的分子基础具有深刻的见解。可用的新隐孢子虫收藏突变体还有助于分子靶标鉴定和药物发现工作。为了方便人们获取信息，在过去的十五年中，通过筛查新孢梭菌收集的大规模基因发现被汇总在一份文件中，并简要介绍了其主要发现。新型隐球菌它可能是唯一具有广泛分析的突变体集合的担子菌酵母，这使其成为创建其他担子菌真菌重要集合的合理模型。实际上，本综述中讨论的全基因组收集代表了该物种毒力中具有相关表型的大量基因，前瞻性筛选可以作为参考点。因此，期望正在进行的研究将从这些全基因组筛选中进行，以鉴定针对真菌疾病的治疗策略。