Background

Allograft contamination during extraction represents a major limiting factor for tissue bank availability. Contamination rates remain persistently high independent of the hospital, country or year considered.

Aim

To analyse the factors associated with contamination of bone and tendon samples extracted from Spanish donors.

Methods

Data for 1162 bone and tendon samples extracted from 102 donors between 2014 and 2017 were collected retrospectively from the hospital database. Descriptive statistics, potentially associated factors and correlation of contamination between samples extracted from different anatomical locations of the same donor were analysed.

Findings

In total, 227 (19.54%) of the extracted samples [131 (18.49%) bone samples and 96 (20.92%) tendon samples] rendered positive cultures and were discarded. Male sex [odds ratio (OR) 2.023; P=0.019], extraction of >10 samples per donor (OR 1.997; P<0.001) and extraction time >240 min (OR 1.755; P=0.001) were factors independently associated with a higher contamination rate. Meanwhile, the tissue sample type ‘bone-patellar tendon-bone’ was associated with a significantly lower contamination rate (OR 0.446; P=0.001). Significant correlation between certain localization of contaminated samples and the concordance of bacterial species was also observed.

Conclusion

Factors related to the extraction procedure, such as total extraction time, extraction sequence, number of samples extracted and anatomical location of extracted samples, play a major role in allograft contamination. Further optimization of procedures, guided by the contamination patterns analysed in this study, should help to increase tissue bank availability.

中文翻译：

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从西班牙捐助者那里购买同种异体骨和腱时细菌污染率和相关因素：探讨污染模式

背景

提取过程中的同种异体移植物污染是组织库可用性的主要限制因素。独立于所考虑的医院，国家或年份，污染率持续居高不下。

目的

分析与西班牙捐赠者抽取的骨骼和肌腱样品污染相关的因素。

方法

从医院数据库中回顾性收集2014年至2017年间从102位捐献者中提取的1162例骨骼和肌腱样本的数据。分析了描述性统计数据，潜在的相关因素以及从同一供体的不同解剖位置提取的样本之间的污染相关性。

发现

总共有227（19.54％）个提取样品[131（18.49％）骨样品和96（20.92％）肌腱样品]呈阳性培养并被丢弃。男性[比值比（OR）2.023；P = 0.019]，每个供体提取10个以上样品（OR 1.997；P <0.001）和提取时间> 240分钟（OR 1.755；P = 0.001）是与较高污染率独立相关的因素。同时，组织样本类型“ b骨腱-骨”与明显较低的污染率相关（OR 0.446；P = 0.001）。还观察到受污染样品的某些定位与细菌物种的一致性之间的显着相关性。

结论

与提取程序有关的因素，例如总提取时间，提取顺序，提取的样本数和提取的样本的解剖位置，在同种异体移植物污染中起主要作用。在这项研究中分析的污染模式的指导下，进一步优化程序应有助于增加组织库的可用性。