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Analysis of RNA polymerase II ubiquitylation and proteasomal degradation
Methods ( IF 4.8 ) Pub Date : 2019-04-01 , DOI: 10.1016/j.ymeth.2019.02.005 Ana Tufegdzic Vidakovic 1 , Michelle Harreman 1 , A Barbara Dirac-Svejstrup 1 , Stefan Boeing 1 , Anindya Roy 1 , Vesela Encheva 2 , Michelle Neumann 1 , Marcus Wilson 1 , Ambrosius P Snijders 2 , Jesper Q Svejstrup 1
Methods ( IF 4.8 ) Pub Date : 2019-04-01 , DOI: 10.1016/j.ymeth.2019.02.005 Ana Tufegdzic Vidakovic 1 , Michelle Harreman 1 , A Barbara Dirac-Svejstrup 1 , Stefan Boeing 1 , Anindya Roy 1 , Vesela Encheva 2 , Michelle Neumann 1 , Marcus Wilson 1 , Ambrosius P Snijders 2 , Jesper Q Svejstrup 1
Affiliation
Highlights • A variety of stimuli, including UV-irradiation, cause RNAPII ubiquitylation and degradation.• Specific considerations are required for reproducible UV-irradiation of human and yeast cells.• Dsk2/MultiDsk pulldown provides robust enrichment of ubiquitylated protein species.• RNAPII degradation level depends on multiple variables.
中文翻译：
RNA聚合酶II泛素化和蛋白酶体降解分析
亮点 • 各种刺激，包括紫外线照射，会导致 RNAPII 泛素化和降解。• 对人类和酵母细胞进行可重复的紫外线照射需要特别考虑。• Dsk2/MultiDsk pulldown 提供了泛素化蛋白质种类的强大富集。• RNAPII 降解水平取决于多个变量。
更新日期：2019-04-01
中文翻译：
RNA聚合酶II泛素化和蛋白酶体降解分析
亮点 • 各种刺激，包括紫外线照射，会导致 RNAPII 泛素化和降解。• 对人类和酵母细胞进行可重复的紫外线照射需要特别考虑。• Dsk2/MultiDsk pulldown 提供了泛素化蛋白质种类的强大富集。• RNAPII 降解水平取决于多个变量。
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