Several key attributes of zebrafish make them an ideal model system for the discovery and development of regeneration promoting therapeutics; most notably their robust capacity for self-repair which extends to the central nervous system. Further, by enabling large-scale drug discovery directly in living vertebrate disease models, zebrafish circumvent critical bottlenecks which have driven drug development costs up. This review summarizes currently available zebrafish phenotypic screening platforms, HTS-ready neurodegenerative disease modeling strategies, zebrafish small molecule screens which have succeeded in identifying regeneration promoting compounds and explores how intravital imaging in zebrafish can facilitate comprehensive analysis of nanocarrier biodistribution and pharmacokinetics. Finally, we discuss the benefits and challenges attending the combination of zebrafish and nanoparticle-based drug optimization, highlighting inspiring proof-of-concept studies and looking toward implementation across the drug development community.

中文翻译：

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让我们变得更小（越来越小）：将斑马鱼和纳米药物结合起来以发展神经再生疗法。

斑马鱼的几个关键属性使其成为发现和开发促进再生疗法的理想模型系统。最值得一提的是，它们具有强大的自我修复能力，可以扩展到中枢神经系统。此外，通过直接在活的脊椎动物疾病模型中实现大规模药物发现，斑马鱼规避了导致药物开发成本上升的关键瓶颈。这篇综述总结了目前可用的斑马鱼表型筛选平台，HTS就绪的神经退行性疾病建模策略，成功鉴定出促进再生化合物的斑马鱼小分子筛选，并探讨了斑马鱼中的活体成像如何促进纳米载体生物分布和药代动力学的综合分析。最后，