Isocitrate dehydrogenase (IDH) I and II mutations in gliomas cause an abnormal accumulation of 2-hydroxyglutarate (2-HG) in these tumor cells. These mutations have potential prognostic value in that knowledge of the mutation status can lead to improved surgical resection. Information on mutation status obtained by immunohistochemistry or genomic analysis is not available during surgery. We report a rapid extraction nanoelectrospray ionization (nESI) method of determining 2-HG. This should allow the determination of IDH mutation status to be performed intraoperatively, within minutes, using a miniature mass spectrometer. This study demonstrates that the combination of tandem mass spectrometry with low-resolution mass spectrometry allows this analysis to be performed with confidence. Graphical Abstract.

中文翻译：

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通过使用微型质谱仪提取纳米电喷雾快速测定人神经胶质瘤的异柠檬酸脱氢酶突变状态。

胶质瘤中的异柠檬酸脱氢酶（IDH）I和II突变会导致这些肿瘤细胞中2-羟基戊二酸（2-HG）异常积聚。这些突变具有潜在的预后价值，因为了解突变状态可以改善手术切除率。在手术期间无法获得通过免疫组织化学或基因组分析获得的突变状态信息。我们报告了确定2-HG的快速提取纳米电喷雾电离（nESI）方法。这应该允许使用微型质谱仪在几分钟内在术中确定IDH突变状态。这项研究表明，串联质谱法与低分辨率质谱法的结合使该分析能够充满信心地进行。图形概要。