当前位置: X-MOL 学术Chem. Sci. › 论文详情
Single quantum dot-based nanosensor for sensitive detection of 5-methylcytosine at both CpG and non-CpG sites
Chemical Science ( IF 9.063 ) Pub Date : 2017-12-13 , DOI: 10.1039/C7SC04813K
Zi-yue Wang, Li-juan Wang, Qianyi Zhang, Bo Tang, Chun-yang Zhang

DNA methylation is an important epigenetic modification in human genomes. Herein, we develop a single quantum dot (QD)-based nanosensor for sensitive detection of DNA methylation at both CpG and non-CpG sites using tricyclic ligation chain reaction (LCR)-mediated QD-based fluorescence resonance energy transfer (FRET). We design two sets of DNA probes (X and Y, X′ and Y′) for methylated DNA assay. In the presence of thermostable DNA ligase, probes X and Y may adjacently hybridize with the methylated DNA to obtain the ligated XY products which may function as the templates for probes X′ and Y′ to generate the X′Y′ products. The resultant X′Y′ products may in turn act as the templates to ligate probes X and Y for the generation of XY products, consequently inducing tricyclic LCR amplification under thermal denaturation conditions to generate a large number of XY products. The subsequent hybridization of XY products with the capture and reporter probes results in the formation of sandwich hybrids which may assemble on the 605QD surface to obtain 605QD–oligonucleotide–Cy5 nanostructures, inducing efficient FRET from the 605QD to Cy5 and the emission of Cy5. This nanosensor can detect DNA methylation at single 5-methylcytosine (5-mC) resolution with a detection limit of as low as 1.0 aM and a large dynamic range of 7 orders of magnitude. Moreover, this nanosensor can distinguish as low as a 0.01% methylation level, and it can detect DNA methylation in human lung cancer cells as well, holding great potential for accurate epigenetic evaluation and early cancer diagnosis.
更新日期:2018-01-31

 

分享到
评论: 0
期刊列表
nature
Nature
Nature
南京大学王少仲课题组招聘有机合成博士后
北京夏禾科技有限公司高薪诚聘有机合成相关研究员
南科大优青团队诚聘高分子/材料化学背景博士后与研究助理
中国科学院化学研究所有机固体实验室江浪研究员课题组招聘有机电子学博士后
中山大学化学工程与技术学院诚聘腐蚀与防护相关专业人才
【问答】请问电活性物质如亚甲基蓝、硫堇等是否可以既被氧化又被还原?
天合科研
2017年中科院JCR分区化学大类列表
试剂库存管理
化合物查询
down
wechat
bug