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Large-scale genomic analyses of in vitro yeast-mycelium dimorphism in human, insect and plant pathogenic fungi: From ESTs to RNAseq experiments
Fungal Biology Reviews ( IF 6 ) Pub Date : 2017-05-05 , DOI: 10.1016/j.fbr.2017.04.001
M. Nigg , L. Bernier

Yeast-mycelium fungal dimorphism is a complex trait studied for many years, particularly in pathogenic fungi for which the morphological switch is often associated with virulence. It is a characteristic shared by many species from very different taxonomic classes. In this review, we present 26 dimorphic fungal species which are pathogens of mammals, insects or plants. Understanding the molecular mechanisms that regulate the morphological switch from yeast to mycelium, or vice-versa, is necessary for the comprehension of virulence. In the last two decades, the development of genome-scale transcriptomic analysis techniques such as Expressed Sequence Tags, DNA microarrays and total messenger RNA sequencing has accelerated the discovery of genes and gene networks that control biological processes. We briefly discuss the three different large-scale transcriptomic techniques, their advantages and disadvantages, and we further detail the studies that used them on seven fungal species in order to understand dimorphism. We compiled the main processes highlighted in these studies and found interesting overlaps with potential conserved molecular regulatory mechanisms among species. With next-generation sequencing technologies becoming increasingly affordable to investigators worldwide, we expect that more exhaustive transcriptomic studies of dimorphism will soon be conducted on a broader range of pathogens including many non-model species.



中文翻译:

人类,昆虫和植物致病真菌中体外酵母菌丝体二态性的大规模基因组分析:从EST到RNAseq实验

酵母菌丝体真菌二态性是一项研究多年的复杂性状,尤其是在病原真菌中,其形态学转换通常与毒力有关。这是来自非常不同的生物分类类别的许多物种共有的一个特征。在这篇综述中,我们介绍了26种双态真菌物种,它们是哺乳动物,昆虫或植物的病原体。理解调节从酵母到菌丝体,或反之亦然的形态转换的分子机制,对于理解毒力是必要的。在过去的二十年中,诸如表达序列标签,DNA微阵列和总信使RNA测序等基因组规模的转录组学分析技术的发展加速了控制生物过程的基因和基因网络的发现。我们简要讨论了三种不同的大规模转录组学技术,它们的优缺点,并且我们进一步详细介绍了将其用于七个真菌物种的研究以了解二态性。我们汇编了这些研究中突出显示的主要过程,发现与物种之间潜在的保守分子调控机制有趣的重叠。随着下一代测序技术越来越为世界各地的研究人员所接受,我们预计不久将对更广泛的病原体,包括许多非典型物种进行更详尽的二态性转录组学研究。我们汇编了这些研究中突出显示的主要过程,并发现了与物种之间潜在的保守分子调控机制有趣的重叠。随着下一代测序技术越来越为世界各地的研究人员所接受,我们预计不久将对更广泛的病原体,包括许多非典型物种进行更详尽的二态性转录组学研究。我们汇编了这些研究中突出显示的主要过程,发现与物种之间潜在的保守分子调控机制有趣的重叠。随着下一代测序技术越来越为世界各地的研究人员所接受,我们预计不久将对更广泛的病原体,包括许多非典型物种进行更详尽的二态性转录组学研究。

更新日期:2017-05-05
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