The octahedral cluster compound Na₂H₈[{Re₆Se₈}(P(C₂H₄CONH₂)(C₂H₄COO)₂)₆] has been shown to be highly radio dense, thus becoming a promising X‐ray contrast agent. It was also shown that this compound had low cytotoxic effect in vitro, low acute toxicity in vivo and was eliminated rapidly from the body through the urinary tract. The present contribution describes a more detailed cellular internalization assay and morphological analysis after intravenous injection of this hexarhenium cluster compound at different doses. The median lethal dose (LD₅₀) of intravenously administrated compound was calculated (4.67 ± 0.69 g/kg). Results of the study clearly indicated that the cluster complex H_n[{Re₆Se₈}(P(C₂H₄CONH₂)(C₂H₄COO)₂)₆]^n–10 was not internalized into cells in vitro and induced only moderate morphological alterations of kidneys at high doses without any changes in morphology of liver, spleen, duodenum, or heart of mice. Copyright © 2016 John Wiley & Sons, Ltd.

中文翻译：

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静脉注射高度亲水的八面体rh簇复合物后的细胞内在化和形态分析–一种新的有前途的X射线造影剂

八面体簇化合物Na ₂ H ₈ [{Re ₆ Se ₈ }（P（C ₂ H ₄ CONH ₂）（C ₂ H ₄ COO）₂）₆ ]已显示出高放射性密度，因此成为很有前途的X射线造影剂。还表明该化合物体外细胞毒作用低，体内急性毒性低。并通过尿路迅速从体内清除。本贡献描述了在以不同剂量静脉注射该六hen簇化合物后的更详细的细胞内化测定和形态分析。计算出静脉内施用化合物的中值致死剂量（LD ₅₀）（4.67±0.69 g / kg）。研究结果清楚地表明，簇复合物H _n [{Re ₆ Se ₈ }（P（C ₂ H ₄ CONH ₂）（C ₂ H ₄ COO）₂）₆ ] ^{n –10}没有内化到细胞中在高剂量下仅在体外和诱导的肾脏中度形态学改变，而小鼠的肝脏，脾脏，十二指肠或心脏的形态没有任何变化。版权所有©2016 John Wiley＆Sons，Ltd.