当前位置: X-MOL 学术Contrast Media Mol. Imaging › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Myelin‐targeted, texaphyrin‐based multimodal imaging agent for magnetic resonance and optical imaging
Contrast Media & Molecular Imaging ( IF 3.009 ) Pub Date : 2016-09-06 , DOI: 10.1002/cmmi.1711
Sashiprabha M. Vithanarachchi 1, 2 , Casey D. Foley 1 , Sarah Trimpin 1 , James R. Ewing 3 , Meser M. Ali 3 , Matthew J. Allen 1
Affiliation  

Reliable methods of imaging myelin are essential to investigate the causes of demyelination and to study drugs that promote remyelination. Myelin‐specific compounds can be developed into imaging probes to detect myelin with various imaging techniques. The development of multimodal myelin‐specific imaging probes enables the use of orthogonal imaging techniques to accurately visualize myelin content and validate experimental results. Here, we describe the synthesis and application of multimodal myelin‐specific imaging agents for light microscopy and magnetic resonance imaging. The imaging agents were synthesized by incorporating the structural features of luxol fast blue MBS, a myelin‐specific histological stain, into texaphyrins coordinated to GdIII. These new complexes demonstrated absorption of visible light, emission of near‐IR light, and relaxivity values greater than clinically approved contrast agents for magnetic resonance imaging. These properties enable the use of optical imaging and magnetic resonance imaging for visualization of myelin. We performed section‐ and en block‐staining of ex vivo mouse brains to investigate the specificity for myelin of the new compounds. Images obtained from light microscopy and magnetic resonance imaging demonstrate that our complexes are retained in white matter structures and enable detection of myelin. Copyright © 2016 John Wiley & Sons, Ltd.

中文翻译:

基于髓磷脂的,基于texaphyrin的多峰成像剂,用于磁共振和光学成像

可靠的髓鞘显像方法对于调查脱髓鞘的原因和研究促进髓鞘再生的药物至关重要。可以将髓磷脂特异性化合物开发为成像探针,以使用各种成像技术来检测髓磷脂。多峰髓鞘特异性成像探针的开发使得可以使用正交成像技术来准确显示髓鞘含量并验证实验结果。在这里,我们描述了用于光学显微镜和磁共振成像的多峰髓鞘特异性显像剂的合成和应用。成像剂是通过将luxel固蓝MBS(一种髓鞘特异性组织学染色剂)的结构特征结合到与Gd III配伍的texaphyrin中而合成的。这些新的复合物表现出的可见光吸收,近红外光的发射以及弛豫度值均大于临床认可的磁共振成像造影剂。这些特性使得可以使用光学成像和磁共振成像对髓磷脂进行可视化。我们对离体小鼠的大脑进行了切片和整块染色,以研究新化合物对髓磷脂的特异性。从光学显微镜和磁共振成像获得的图像表明,我们的复合物保留在白质结构中,并能够检测髓磷脂。版权所有©2016 John Wiley&Sons,Ltd.
更新日期:2016-09-06
down
wechat
bug