Implant-associated bacterial infections pose serious medical and financial issues due to the colonization and proliferation of pathogens on the surface of the implant. The as-prepared traditional antibacterial surfaces can neither resist bacterial adhesion nor inhibit the development of biofilm over the long term. Herein, novel (montmorillonite/poly-L-lysine-gentamicin sulfate)₈ ((MMT/PLL-GS)₈) organic–inorganic hybrid multilayer films were developed to combine enzymatic degradation PLL for on-demand self-defense antibiotics release. Small molecule GS was loaded into the multilayer films during self-assembly and the multilayer films showed pH-dependent and linear growth behavior. The chymotrypsin- (CMS) and bacterial infections-responsive film degradation led to the peeling of the films and GS release. Enzyme-responsive GS release exhibited CMS concentration dependence as measured by the size of the inhibition zone and SEM images. Notably, the obtained antibacterial films showed highly efficient bactericidal activity which killed more than 99.9% of S. aureus in 12 h. Even after 3 d of incubation in S. aureus, E. coli or S. epidermidis solutions, the multilayer films exhibited inhibition zones of more than 1.5 mm in size. Both in vitro and in vivo antibacterial tests indicated good cell compatibility, and anti-inflammatory, and long-term bacterial anti-adhesion and biofilm inhibition properties.

中文翻译：

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从有机-无机杂化多层膜中释放细菌自卫抗生素，具有长期抗粘附和生物膜抑制特性

由于病原体在植入物表面的定植和增殖，与植入物相关的细菌感染会带来严重的医疗和财务问题。所制备的传统抗菌表面既不能抵抗细菌粘附，也不能长期抑制生物膜的形成。在此，开发了新型（蒙脱土/聚-L-赖氨酸-庆大霉素硫酸盐）₈（（MMT/PLL-GS）₈）有机-无机杂化多层膜，将酶促降解PLL结合起来，用于按需释放自卫抗生素。在自组装过程中将小分子 GS 加载到多层膜中，多层膜表现出 pH 依赖性和线性生长行为。胰凝乳蛋白酶（CMS）和细菌感染响应性膜降解导致膜剥落和 GS 释放。通过抑制区的大小和 SEM 图像测量，酶响应 GS 释放表现出 CMS 浓度依赖性。值得注意的是，所获得的抗菌膜表现出高效的杀菌活性，12小时内杀死99.9%以上的金黄色葡萄球菌。即使在金黄色葡萄球菌、大肠杆菌或表皮葡萄球菌溶液中孵育3天后，多层膜仍表现出尺寸超过1.5毫米的抑制区。体外和体内抗菌测试均显示出良好的细胞相容性、抗炎、长期细菌抗粘附和生物膜抑制特性。