Despite the potential sensitivity and the wide range of applications for surface-enhanced Raman spectroscopy (SERS), it is not used as a routine detection tool due mainly to the poor reproducibility of the enhanced SERS signals. In order to obtain reproducibly strong SERS data, both lithographic and non-lithographic approaches are intensively investigated to produce large-area nanopatterned SERS substrates displaying periodically arranged arrays of nanostructures. Herein, we report a facile method for the non-lithographic fabrication of plasmonic nanoparticle arrays by utilizing the barrier sides of anodized aluminum oxide (AAO) membranes. The nanobump-decorated surfaces (NBDS) of AAO barrier sides were treated with wet etching to create periodic arrays of nanocraters. Upon coating with an optimized thickness of Au, the nanocrater decorated surfaces (NCDS) displayed intensified SERS signals compared with the NBDS counterparts. This result was also confirmed with simulation studies and it was related to the increased surface roughness for the NCDS substrates. The fabricated [email protected] nanoplatforms were stable for extended periods and allowed enhanced and reproducible SERS signals with relative standard deviation values ∼10% from independently prepared samples. Our current studies are focused on the potential use of these SERS substrates for sensing biomarker molecules including myoglobin and troponin-T.

尽管表面增强拉曼光谱法（SERS）具有潜在的灵敏度和广泛的应用范围，但由于增强的SERS信号的重现性较差，因此不能将其用作常规检测工具。为了获得可再现的强SERS数据，对光刻和非光刻方法都进行了深入研究，以生产显示出周期性排列的纳米结构阵列的大面积纳米图案SERS基板。在这里，我们报告了一种利用阳极氧化铝（AAO）膜的势垒面非平版印刷等离子纳米粒子阵列的简便方法。用湿法蚀刻处理AAO势垒面的纳米凸点装饰表面（NBDS），以创建纳米坑的周期性阵列。在镀上优化厚度的金之后，与NBDS对应物相比，纳米坑装饰面（NCDS）显示出增强的SERS信号。模拟研究也证实了这一结果，这与NCDS基材表面粗糙度的增加有关。所制造的[受电子邮件保护的]纳米平台可以长期稳定，并且可以从独立制备的样品中获得相对标准偏差值为〜10％的增强且可再现的SERS信号。我们目前的研究集中在这些SERS底物在检测包括肌红蛋白和肌钙蛋白T在内的生物标志物分子方面的潜在用途。所制造的[受电子邮件保护的]纳米平台可以长期稳定，并且可以从独立制备的样品中获得相对标准偏差值为〜10％的增强且可再现的SERS信号。我们目前的研究集中在这些SERS底物在检测包括肌红蛋白和肌钙蛋白T在内的生物标志物分子方面的潜在用途。所制造的[受电子邮件保护的]纳米平台可以长期稳定，并且可以从独立制备的样品中获得相对标准偏差值为〜10％的增强且可再现的SERS信号。我们目前的研究集中在这些SERS底物在检测包括肌红蛋白和肌钙蛋白T在内的生物标志物分子方面的潜在用途。