A new approach that uses a hybrid Q-FTICR instrument and combines quadrupole collision-induced dissociation, hydrogen-deuterium exchange, and infrared multiphoton dissociation (QCID-HDX-IRMPD) has been shown to effectively separate and differentiate isomeric fragment ion structures present at the same m/z. This method was used to study protonated YAGFL-OH (free acid), YAGFL-NH₂ (amide), cyclic YAGFL, and YAGFL-OCH₃ (methyl ester). QCID-HDX of m/z 552.28 (C₂₉H₃₈N₅O₆) from YAGFL-OH reveals at least two distributions of ions corresponding to the b₅ ion and a non-C-terminal water loss ion structure. Subsequent IRMPD fragmentation of each population shows distinct fragmentation patterns, reflecting the different structures from which they arise. This contrasts with data for YAGFL-NH₂ and YAGFL-OCH₃, which do not show two distinct H/D exchange populations for the C₂₉H₃₈N₅O₆ structure formed by NH₃ and HOCH₃ loss, respectively. Relative extents of exchange for C₂₉H₃₈N₅O₆ ions from six sequence isomers (YAGFL, AGFLY, GFLYA, FLYAG, LYAGF, and LFGAY) show a sequence dependence of relative isomer abundance. Supporting action IRMPD spectroscopy data are also presented herein and also show that multiple structures are present for the C₂₉H₃₈N₅O₆ species from YAGFL-OH.

一种使用混合 Q-FTICR 仪器并结合四极杆碰撞诱导解离、氢-氘交换和红外多光子解离 (QCID-HDX-IRMPD) 的新方法已被证明可以有效地分离和区分存在于相同的m/z。该方法用于研究质子化的 YAGFL-OH（游离酸）、YAGFL-NH ₂（酰胺）、环状 YAGFL 和 YAGFL-OCH ₃（甲酯）。来自 YAGFL-OH的m/z 552.28 (C ₂₉ H ₃₈ N ₅ O ₆ ) 的QCID-HDX揭示了至少两种对应于 b ₅的离子分布离子和非 C 端失水离子结构。每个种群的后续 IRMPD 碎裂显示出不同的碎裂模式，反映了它们产生的不同结构。这与YAGFL-NH ₂和YAGFL-OCH _{3 的}数据形成对比，YAGFL-NH ₂和YAGFL-OCH ₃没有显示分别由NH ₃和HOCH ₃损失形成的C ₂₉ H ₃₈ N ₅ O ₆结构的两个不同的H/D交换群。C ₂₉ H ₃₈ N ₅ O _{6 的}相对交换程度来自六个序列异构体（YAGFL、AGFLY、GFLYA、FLYAG、LYAGF 和 LFGAY）的离子显示相对异构体丰度的序列依赖性。支持作用IRMPD光谱数据也在本文中提供并且还表明来自YAGFL-OH的C ₂₉ H ₃₈ N ₅ O ₆物质存在多种结构。