Annual Review of Analytical Chemistry ( IF 8 ) Pub Date : 2017-06-12 00:00:00 , DOI: 10.1146/annurev-anchem-061516-045317 Aleem Syed 1, 2 , Emily A. Smith 1, 2
Raman-based optical imaging is a promising analytical tool for noninvasive, label-free chemical imaging of lipid bilayers and cellular membranes. Imaging using spontaneous Raman scattering suffers from a low intensity that hinders its use in some cellular applications. However, developments in coherent Raman imaging, surface-enhanced Raman imaging, and tip-enhanced Raman imaging have enabled video-rate imaging, excellent detection limits, and nanometer spatial resolution, respectively. After a brief introduction to these commonly used Raman imaging techniques for cell membrane studies, this review discusses selected applications of these modalities for chemical imaging of membrane proteins and lipids. Finally, recent developments in chemical tags for Raman imaging and their applications in the analysis of selected cell membrane components are summarized. Ongoing developments toward improving the temporal and spatial resolution of Raman imaging and small-molecule tags with strong Raman scattering cross sections continue to expand the utility of Raman imaging for diverse cell membrane studies.
中文翻译：
细胞膜，富含脂质的细胞器和脂质双层中的拉曼成像
基于拉曼的光学成像是一种有前景的分析工具，可用于脂质双层和细胞膜的无创，无标签化学成像。使用自发拉曼散射的成像强度低，阻碍了其在某些细胞应用中的使用。但是，相干拉曼成像，表面增强拉曼成像和尖端增强拉曼成像的发展分别使视频速率成像，出色的检测极限和纳米空间分辨率成为可能。在简要介绍了用于细胞膜研究的这些常用拉曼成像技术后，本篇综述讨论了这些方法在膜蛋白和脂质化学成像中的某些应用。最后，总结了拉曼成像化学标签的最新发展及其在所选细胞膜成分分析中的应用。正在进行的改善拉曼成像和具有强拉曼散射横截面的小分子标签的时间和空间分辨率的发展，继续扩大了拉曼成像在各种细胞膜研究中的用途。
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