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SARS-Cov-2 Spike-S1 Antigen Test Strip with High Sensitivity Endowed by High-Affinity Antibodies and Brightly Fluorescent QDs/Silica Nanospheres
ACS Applied Materials & Interfaces ( IF 9.5 ) Pub Date : 2023-06-02 , DOI: 10.1021/acsami.3c03434
Shiyi Tao 1, 2 , Xiaomin Zhao 1 , Dongping Bao 2 , Xuecheng Liu 1 , Wei Zhang 3 , Liying Zhao 3 , Yujiao Tang 1 , Hongbin Wu 4 , Huayue Ye 4 , Yili Yang 2 , Dawei Deng 1, 3
Affiliation  

The extensive research into developing novel strategies for detecting respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens in clinical specimens, especially the sensitive point-of-care testing method, is still urgently needed to reach rapid screening of viral infections. Herein, a new lateral flow immunoassay (LFIA) platform was reported for the detection of SARS-CoV-2 spike-S1 protein antigens, in which four sensitive and specific SARS-CoV-2 mouse monoclonal antibodies (MmAbs) were tailored by using quantum dot (QD)-loaded dendritic mesoporous silica nanoparticles modified further for achieving the −COOH group surface coating (named Q/S–COOH nanospheres). Importantly, compact QD adsorption was achieved in mesoporous channels of silica nanoparticles on account of highly accessible central–radial pores and electrostatic interactions, leading to significant signal amplification. As such, a limit of detection for SARS-CoV-2 spike-S1 testing was found to be 0.03 ng/mL, which is lower compared with those of AuNPs-LFIA (traditional colloidal gold nanoparticles, Au NPs) and enzyme-linked immunosorbent assay methods. These results show that optimizing the affinity of antibody and the intensity of fluorescent nanospheres simultaneously is of great significance to improve the sensitivity of LFIA.

中文翻译:

高亲和力抗体和明亮荧光量子点/二氧化硅纳米球赋予的高灵敏度 SARS-Cov-2 Spike-S1 抗原测试条

仍然迫切需要广泛研究开发检测临床标本中呼吸综合征冠状病毒 2 (SARS-CoV-2) 抗原的新策略,特别是敏感的即时检测方法,以实现病毒感染的快速筛查。本文报道了一种新的侧流免疫分析(LFIA)平台,用于检测 SARS-CoV-2 刺突 S1 蛋白抗原,其中通过使用量子技术定制了四种敏感且特异的 SARS-CoV-2 小鼠单克隆抗体(MmAb)点(QD)负载的树枝状介孔二氧化硅纳米粒子经过进一步修饰以实现-COOH基团表面涂层(称为Q/S-COOH纳米球)。重要的是,由于易于接近的中心径向孔和静电相互作用,在二氧化硅纳米颗粒的介孔通道中实现了紧凑的量子点吸附,从而导致显着的信号放大。因此,SARS-CoV-2 spike-S1 检测的检测限为 0.03 ng/mL,低于 AuNPs-LFIA(传统胶体金纳米粒子,Au NPs)和酶联免疫吸附剂的检测限测定方法。这些结果表明,同时优化抗体的亲和力和荧光纳米球的强度对于提高LFIA的灵敏度具有重要意义。
更新日期:2023-06-02
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