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Combined transcriptomic and targeted metabolomic analysis reveals the mechanism of flesh browning in cold stored ‘Fuji’ apple fruit
Scientia Horticulturae ( IF 4.3 ) Pub Date : 2023-06-01 , DOI: 10.1016/j.scienta.2023.112195
Jihan Wang, Fujun Li, Xinyue Zhang, Wenen Sun, Maratab Ali, Xiaoan Li, Xinhua Zhang

Flesh browning (FB) is a common physiological disease of apple fruit during cold storage, which physiological mechanism was revealed in the present study through analyzing physical, transcriptomic, and targeted metabolomic parameters. A total of 8559 differentially expressed genes were identified between FB and normal flesh. In FB, the glycolysis/gluconeogenesis pathway was down-regulated; starch and sucrose metabolism, phenylpropane biosynthesis, plant hormone signal transduction, mitogen-activated protein kinase signaling pathway, and plant-pathogen interaction were all up-regulated. In addition, FB was also associated with the expression level of genes related to defense, cold stress, and reactive oxygen species (ROS) scavenging, and various transcription factor families. Characteristics of FB tissue included lower contents of flavonoids and sugars, higher contents of jasmonic acid and ROS, lower organic acids contents and higher proportion of lactic acid/malic acid level. In conclusion, FB is a complex metabolic process, which involves the changes in the metabolites, genes expression, and transcriptional regulations in the primary metabolism, secondary metabolism, signal transduction and other pathways of apple fruit during cold storage.



中文翻译:

结合转录组学和靶向代谢组学分析揭示冷藏“富士”苹果果实果肉褐变的机制

果肉褐变(FB)是苹果果实在冷藏过程中常见的生理病害,本研究通过物理、转录组和靶向代谢组学参数分析揭示了其生理机制。在 FB 和正常肉之间共鉴定出 8559 个差异表达基因。在 FB 中,糖酵解/糖异生途径被下调;淀粉和蔗糖代谢、苯丙烷生物合成、植物激素信号转导、丝裂原活化蛋白激酶信号通路和植物-病原体相互作用均上调。此外,FB 还与防御、冷应激和活性氧 (ROS) 清除相关基因的表达水平以及各种转录因子家族有关。FB组织的特征是黄酮类和糖类含量较低,茉莉酸和ROS含量较高,有机酸含量较低,乳酸/苹果酸水平比例较高。综上所述,FB是一个复杂的代谢过程,涉及苹果果实在冷藏过程中初级代谢、次级代谢、信号转导等通路中代谢产物、基因表达、转录调控等方面的变化。

更新日期:2023-06-02
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