Benzoic acid (BA), a secondary metabolite released through root exudates, is considered to be the most common inhibitor that leads to plant autotoxicity, even at low concentrations in closed hydroponic systems. In this study, to mitigate BA-driven autotoxicity, the effects of O₃ and O₃/H₂O₂ oxidation treatment (O₃ concentration: 1, 2, 4, 8 mg L⁻¹, H₂O₂ concentration: 4, 8 mg L⁻¹) on waste nutrient solution (WNS) were investigated in terms of BA degradation, the rate of germination inhibition (GI), and the rate of root growth inhibition (RI). In the case of O₃ treatment, the BA degradation rate improved up to 14.1% as the O₃ concentration increased, while alleviation of GI was insignificant (94.6–100%), confirming that a single O₃ treatment was unsuitable for mitigating autotoxicity. On the other hand, O₃/H₂O₂ treatment increased BA degradation by up to 24.8%, thereby significantly reducing GI (up to 7.69%) and RI (up to 0.88%). Both the highest BA mineralization rate and phytotoxicity mitigation was observed at BA₁₂₅ (4–4) (BA mineralization: 16.7%, GI: 12.82%, RI: 11.69%) and BA₁₂₅ (1–8) (BA mineralization: 17.7%, GI: 7.69%, RI: 0.88%) at each H₂O₂ concentration. In addition, the operating costs were evaluated by a chemical and electricity cost analysis at the different treatments. As a result, the operating costs of BA₁₂₅ (4–4) and BA₁₂₅ (1–8) were calculated to be 0.40 and 0.42 $ L⁻¹ mg⁻¹ of mineralized BA, respectively. After consideration of the mineralization rate, autotoxicity mitigation, and operating cost, BA₁₂₅ (1–8) was suggested for the optimal treatment condition and our findings would contribute to the alleviation of BA-driven autotoxicity.

苯甲酸 (BA) 是一种通过根系分泌物释放的次级代谢产物，被认为是导致植物自毒的最常见抑制​​剂，即使在封闭的水培系统中浓度很低也是如此。_{在这项研究中，为了减轻 BA 驱动的自毒性，O 3}和 O ₃ /H ₂ O ₂氧化处理的影响_{（O 3}浓度：1, 2, 4, 8 mg L ^-1，H ₂ O ₂浓度：4 , 8 mg L ^-1 ) 对废营养液 (WNS) 的 BA 降解、发芽抑制率 (GI) 和根系生长抑制率 (RI) 进行了研究。_{在 O 3}的情况下_{处理后，随着 O 3}浓度的增加，BA 降解率提高至 14.1% ，而 GI 的缓解不显着 (94.6–100%)，证实单次 O ₃处理不适合减轻自身毒性。另一方面，O ₃ /H ₂ O ₂处理使 BA 降解增加高达 24.8%，从而显着降低 GI（高达 7.69%）和 RI（高达 0.88%）。_{在 BA 125} (4–4)（BA 矿化：16.7%，GI：12.82%，RI：11.69%）和 BA ₁₂₅ (1–8)（BA 矿化：17.7% ）观察到最高的 BA 矿化率和植物毒性缓解, GI: 7.69%, RI: 0.88%) 在每个 H ₂ O ₂专注。此外，通过对不同处理的化学和电力成本分析来评估运营成本。结果，计算得出BA ₁₂₅ (4–4) 和 BA _{125 (1–8) 的运营成本分别为 0.40 和 0.42 $ L} ^-1 mg ^-1矿化 BA。在考虑矿化率、自毒性缓解和运营成本后，建议使用BA _{125 (1–8) 作为最佳处理条件，我们的研究结果将有助于减轻 BA 驱动的自毒性。}