Persistent luminescence (PersL) nanoparticles (PLNPs) with excitation-afterglow emission separation character, have brought luciferous prospect for ultrasensitive optical detection to circumvent the weakness of clinical fluorescence modality, benefiting from the strengths of autofluorescence-free, low phototoxicity and superior photostability. Nevertheless, small dimensions of PLNPs (<200 nm, suitable for in vivo diagnosis) synthesized via current procedures are inevitably accompanied with PersL brightness sacrifice. Whereas the past few years have witnessed advances to promote the performance of nanophosphor, proposing a versatile and sustainable approach to boost the excitation absorption capacity, thus amplifying the PersL is of industrywide urgency. Here, we first report a “lanthanide-chelate sensitization” strategy involving Eu-DOTA as strong photon harvester and Zn₂SnO₄: Cr³⁺, Eu³⁺ (ZSCE) PLNPs as matched acceptor to steadily augment the intensity of obtained ZDE PLNPs via efficacious Förster-type energy transfer. Photobleaching resistant ZDE PLNPs with different DOTA-Eu substitutions enable maximal 3.9-fold luminous brightness and 1.4-fold decay lifetime over bare PLNPs. Cladded with hyaluronic acid (HA), ZDE@HA PLNPs performed high signal-to-noise ratio imaging and accurate tumors boundary depicting which facilitates PersL-guided breast tumors excision. Strikingly, the “Ln-chelate sensitization” concept could serve as an extensible approach to optimize existing PLNPs, which lightens up new perspectives for ultra-contrastive malignancy navigation.

具有激发-余辉发射分离特性的持久发光 (PersL) 纳米粒子 (PLNPs) 具有无自发荧光、低光毒性和优异的光稳定性等优点，为超灵敏光学检测带来了光明的前景，克服了临床荧光模式的弱点。然而，通过合成的小尺寸 PLNPs（<200 nm，适用于体内诊断）当前的程序不可避免地伴随着 PersL 亮度牺牲。鉴于过去几年见证了提高纳米荧光粉性能的进步，提出一种通用且可持续的方法来提高激发吸收能力，从而放大 PersL 是整个行业的当务之急。在这里，我们首先报告了一种“镧系元素螯合物敏化”策略，涉及 Eu-DOTA 作为强光子收集器和 Zn ₂ SnO ₄ : Cr ³⁺ , Eu ³⁺ (ZSCE) PLNPs 作为匹配受体以稳步增加获得的 ZDE PLNPs 的强度通过有效的 Förster 型能量转移。具有不同 DOTA-Eu 取代的抗光漂白 ZDE PLNPs 与裸 PLNPs 相比，可实现最大 3.9 倍的发光亮度和 1.4 倍的衰变寿命。包覆透明质酸 (HA) 的 ZDE@HA PLNPs 进行了高信噪比成像和准确的肿瘤边界描绘，这有助于 PersL 引导的乳腺肿瘤切除。引人注目的是，“Ln-螯合物敏化”概念可以作为优化现有 PLNPs 的可扩展方法，从而为超对比恶性肿瘤导航开辟了新的视角。