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FvWRKY50 is an important gene that regulates both vegetative growth and reproductive growth in strawberry
Horticulture Research ( IF 8.7 ) Pub Date : 2023-05-31 , DOI: 10.1093/hr/uhad115 Yating Chen 1 , Liping Liu 1 , Qianqian Feng 1 , Chuang Liu 1 , Yujuan Bao 1 , Nan Zhang 1 , Ronghui Sun 1 , Zhaonan Yin 1 , Chuanfei Zhong 2 , Yuanhua Wang 3, 4 , Qian Li 1 , Bingbing Li 1
Horticulture Research ( IF 8.7 ) Pub Date : 2023-05-31 , DOI: 10.1093/hr/uhad115 Yating Chen 1 , Liping Liu 1 , Qianqian Feng 1 , Chuang Liu 1 , Yujuan Bao 1 , Nan Zhang 1 , Ronghui Sun 1 , Zhaonan Yin 1 , Chuanfei Zhong 2 , Yuanhua Wang 3, 4 , Qian Li 1 , Bingbing Li 1
Affiliation
WRKY transcription factor plays important roles in plant growth and resistance, but only a few members have been identified in strawberry. Here we identified a WRKY transcription factor FvWRKY50 in diploid strawberry which played essential roles in strawberry vegetative growth and reproductive growth. Knocking out FvWRKY50 by genome editing accelerated flowering time and leaf senescence but delayed anthocyanin accumulation in fruit. Further analysis showed that FvWRKY50 acted as transcriptional repressor to negatively regulate the expression of flowering and leaf senescence related genes including FvFT2, FvCO, FvFT3 and FvSAUR36. Notably, FvWRKY50 directly upregulated the expression of FvCHI and FvDFR by binding their promoter under normal condition, but at low temperature FvWRKY50 was phosphorylated by FvMAPK3 and then induced protein degradation by ubiquitination, delaying anthocyanin accumulation. In addition, the homozygous mutant of FvWRKY50 was smaller while the biallelic mutant showed normal size. These new findings provide important clues for us to further reveal the regulatory mechanisms of strawberry growth and fruit ripening.
中文翻译:
FvWRKY50是调节草莓营养生长和生殖生长的重要基因
WRKY转录因子在植物生长和抗逆性中发挥着重要作用,但在草莓中仅鉴定了少数成员。在这里,我们在二倍体草莓中鉴定了一个WRKY转录因子FvWRKY50,它在草莓的营养生长和生殖生长中发挥着重要作用。通过基因组编辑敲除 FvWRKY50 可加速开花时间和叶子衰老,但会延迟果实中花青素的积累。进一步分析表明,FvWRKY50作为转录阻遏蛋白,负向调节开花和叶片衰老相关基因的表达,包括FvFT2、FvCO、FvFT3和FvSAUR36。值得注意的是,FvWRKY50在正常条件下通过结合FvCHI和FvDFR的启动子直接上调FvCHI和FvDFR的表达,但在低温下FvWRKY50被FvMAPK3磷酸化,然后通过泛素化诱导蛋白质降解,延迟花青素的积累。此外,FvWRKY50的纯合突变体较小,而双等位突变体则显示正常大小。这些新发现为我们进一步揭示草莓生长和果实成熟的调控机制提供了重要线索。
更新日期:2023-05-31
中文翻译:
FvWRKY50是调节草莓营养生长和生殖生长的重要基因
WRKY转录因子在植物生长和抗逆性中发挥着重要作用,但在草莓中仅鉴定了少数成员。在这里,我们在二倍体草莓中鉴定了一个WRKY转录因子FvWRKY50,它在草莓的营养生长和生殖生长中发挥着重要作用。通过基因组编辑敲除 FvWRKY50 可加速开花时间和叶子衰老,但会延迟果实中花青素的积累。进一步分析表明,FvWRKY50作为转录阻遏蛋白,负向调节开花和叶片衰老相关基因的表达,包括FvFT2、FvCO、FvFT3和FvSAUR36。值得注意的是,FvWRKY50在正常条件下通过结合FvCHI和FvDFR的启动子直接上调FvCHI和FvDFR的表达,但在低温下FvWRKY50被FvMAPK3磷酸化,然后通过泛素化诱导蛋白质降解,延迟花青素的积累。此外,FvWRKY50的纯合突变体较小,而双等位突变体则显示正常大小。这些新发现为我们进一步揭示草莓生长和果实成熟的调控机制提供了重要线索。