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Unveiling the spatial distribution and molecular mechanisms of terpenoids biosynthesis in salvia miltiorrhiza and S. grandifolia using multi-omics and DESI-MSI
Horticulture Research ( IF 8.7 ) Pub Date : 2023-05-31 , DOI: 10.1093/hr/uhad109
Jie Xia 1 , Ganggui Lou 1 , Lan Zhang 1 , Yanbo Huang 2 , Jian Yang 3 , Juan Guo 3 , Zhechen Qi 1 , Zhenhao Li 4 , Guoliang Zhang 4 , Shengchun Xu 5 , Xijiao Song 5 , Xiaodan Zhang 1 , Yukun Wei 6 , Zongsuo Liang 1 , Dongfeng Yang 1
Affiliation  

Salvia miltiorrhiza and S. grandifolia are rich in diterpenoids and have therapeutic effects on cardiovascular diseases. In this study, the spatial distribution of diterpenoids in both species was analyzed by a combination of metabolomics and mass spectrometry imaging techniques. The result indicated that diterpenoids in S. miltiorrhiza were mainly abietane-type norditerpenoid quinones with a furan or dihydrofuran D-ring, they were mainly distributed in the periderm of the roots, such as cryptotanshinone and tanshinone IIA. The compounds in S. grandifolia were mainly phenolic abietane-type tricyclic diterpenoids with six- or seven-membered C-rings, and were widely distributed in the periderm, phloem and xylem of the roots such as 11-hydroxy-sugiol, 11,20-dihydroxy-sugiol and 11,20-dihydroxy-ferruginoll. In addition, the leaves of S. grandifolia were rich in tanshinone biosynthesis precursors, such as 11-hydroxy-sugiol, while those of S. miltiorrhiza were rich in phenolic acids. Genes in the upstream pathway of tanshinone biosynthesis are highly expressed in the root of S. grandifolia, and genes in the downstream pathway are highly expressed in the root of S. miltiorrhiza. Here, we describe the specific tissue distributions and mechanism of diterpenoids in two Salvia species, which will facilitate further investigations of the biosynthesis of diterpenoids in plant synthetic biology.

中文翻译:

利用多组学和 DESI-MSI 揭示丹参和大叶丹参中萜类生物合成的空间分布和分子机制

丹参、大叶丹参富含二萜类化合物,对心血管疾病有治疗作用。在这项研究中,通过代谢组学和质谱成像技术的结合分析了两个物种中二萜类化合物的空间分布。结果表明,丹参中的二萜类化合物主要为含呋喃或二氢呋喃D环的松香烷型去甲二萜醌类化合物,主要分布在根周皮,如隐丹参酮、丹参酮IIA等。大叶槐中的化合物主要为六元或七元C环的酚类松香烷型三环二萜类化合物,广泛分布于根的周皮、韧皮部和木质部,如11-羟基-杉醇、11,20等。 -二羟基-sugiol和11,20-二羟基-ferruginoll。此外,大叶丹参的叶子富含丹参酮生物合成前体,如11-羟基杉醇,而丹参的叶子则富含酚酸。丹参酮生物合成上游途径基因在大叶丹参根中高表达,下游途径基因在丹参根中高表达。在这里,我们描述了两种丹参中二萜类化合物的特定组织分布和机制,这将有助于进一步研究植物合成生物学中二萜类化合物的生物合成。
更新日期:2023-05-31
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