Centrioles are critical for fundamental cellular processes, including signaling, motility, and division. The extent to which centrioles are present after cell cycle exit in a developing organism is not known. The stereotypical lineage of Caenorhabditis elegans makes it uniquely well-suited to investigate this question. Using notably lattice light-sheet microscopy, correlative light electron microscopy, and lineage assignment, we found that ~88% of cells lose centrioles during embryogenesis. Our analysis reveals that centriole elimination is stereotyped, occurring invariably at a given time in a given cell type. Moreover, we established that experimentally altering cell fate results in corresponding changes in centriole fate. Overall, we uncovered the existence of an extensive centriole elimination program, which we anticipate to be paradigmatic for a broad understanding of centriole fate regulation.

中文翻译：

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线虫胚胎中发现了广泛的程序性中心粒消除

中心粒对于基本细胞过程至关重要，包括信号传导、运动和分裂。在发育中的生物体中，细胞周期退出后中心粒存在的程度尚不清楚。典型的血统秀丽隐杆线虫使其非常适合研究这个问题。特别是使用晶格光片显微镜、相关光电子显微镜和谱系分配，我们发现约 88% 的细胞在胚胎发生过程中失去中心粒。我们的分析表明，中心粒消除是固定的，在给定的细胞类型中总是在给定的时间发生。此外，我们确定通过实验改变细胞命运会导致中心粒命运的相应变化。总的来说，我们发现了广泛的中心粒消除计划的存在，我们预计这将成为对中心粒命运调控的广泛理解的范例。