The transport, distribution, and mixing of microfluidics often require additional instruments, such as pumps and valves, which are not feasible when operated in point-of-care (POC) settings. Here, we present a simple microfluidic pathogen detection system known as Rotation-Chip that transfers the reagents between wells by manually rotating two concentric layers without using external instruments. The Rotation-Chip is fabricated by a simple computer numerical control (CNC) machining process and is capable of carrying out 60 multiplexed reactions with a simple 30 or 60° rotation. Leveraging superhydrophobic coating, a high fluid transport efficiency of 92.78% is achieved without observable leaking. Integrated with an intracellular fluorescence assay, an on-chip detection limit of 1.8 × 10⁶ CFU/mL is achieved for ampicillin-resistant Escherichia coli (E. coli), which is similar to our off-chip results. We also develop a computer vision method to automatically distinguish positive and negative samples on the chip, showing 100% accuracy. Our Rotation-Chip is simple, low-cost, high-throughput, and can display test results with a single chip image, making it ideal for various multiplexing POC applications in resource-limited settings.

中文翻译：

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用于耐药细菌计算机视觉鉴定的超疏水旋转芯片

微流体的传输、分配和混合通常需要额外的仪器，例如泵和阀门，这在即时护理 (POC) 环境中操作时是不可行的。在这里，我们提出了一个简单的微流体病原体检测系统，称为旋转芯片，通过手动旋转两个同心层在孔之间转移试剂，而无需使用外部仪器。旋转芯片是通过简单的计算机数控 (CNC) 加工工艺制造的，能够通过简单的 30 或 60° 旋转进行 60 次多重反应。利用超疏水涂层，实现了 92.78% 的高流体传输效率，而没有明显的泄漏。集成细胞内荧光检测，片上检测限为 1.8 × 10 ⁶抗氨苄青霉素大肠杆菌 (E. coli)达到了 CFU/mL ，这与我们的芯片外结果相似。我们还开发了一种计算机视觉方法，可以自动区分芯片上的正负样本，显示出 100% 的准确率。我们的 Rotation-Chip 简单、低成本、高吞吐量，并且可以用单芯片图像显示测试结果，非常适合资源有限环境中的各种多路复用 POC 应用。