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Antimicrobial activity and mechanism of Magnolia officinalis root extract against methicillin-resistant Staphylococcus aureus based on mannose transporter
Industrial Crops and Products ( IF 5.9 ) Pub Date : 2023-05-30 , DOI: 10.1016/j.indcrop.2023.116953
Jingwen Sun, Yu Xie, Zhiyuan Chen, Yipin Fan, Yunfeng Liu, Qi Gao, Jianqiang Li, Jingwen Bai, Yu Yang

Magnolia officinalis root extract was obtained by high-pressure-assisted extraction, and the yield of honokiol (HNK) was 86.97 ± 1.05 mg/g dry power. The purified extracts and HNK inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA). Assays for alkaline phosphatases, proteins, nucleic acids leakage, and electrical conductivity, along with scanning and transmission electron microscopy measurements, confirmed the damaging effects of HNK on the cell wall and membrane of MRSA. Intake of sugar, protein production, molecular docking, and dynamic simulations revealed that HNK interfered with the transport of mannose-based carbohydrates by binding to VAL196, ILE291, ALA293, PRO294, and MET214 on the mannose transporter (ManP) in the phosphotransferase system, and thereby inhibited the carbohydrate metabolism of MRSA. These results indicated that HNK prevented MRSA from using carbohydrates by cutting off its nutritional process, and ultimately caused bacterial rupture and lysis. Therefore, ManP may be a potential target for HNK to combat MRSA infections.



中文翻译:

基于甘露糖转运蛋白的厚朴根提取物对耐甲氧西林金黄色葡萄球菌的抑菌活性及机制

通过高压辅助提取获得厚朴根提取物,和厚朴酚(HNK)的得率为86.97±1.05mg/g干粉。纯化的提取物和 HNK 抑制耐甲氧西林金黄色葡萄球菌的生长(耐甲氧西林金黄色葡萄球菌)。碱性磷酸酶、蛋白质、核酸渗漏和电导率的测定,以及扫描和透射电子显微镜测量,证实了 HNK 对 MRSA 细胞壁和细胞膜的破坏作用。糖的摄入、蛋白质生产、分子对接和动态模拟表明,HNK 通过与磷酸转移酶系统中甘露糖转运蛋白 (ManP) 上的 VAL196、ILE291、ALA293、PRO294 和 MET214 结合,干扰基于甘露糖的碳水化合物的转运,从而抑制了MRSA的碳水化合物代谢。这些结果表明,HNK 通过切断其营养过程来阻止 MRSA 使用碳水化合物,并最终导致细菌破裂和裂解。因此,ManP 可能是 HNK 对抗 MRSA 感染的潜在目标。

更新日期:2023-05-31
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