Journal of Hazardous Materials ( IF 13.6 ) Pub Date : 2023-05-31 , DOI: 10.1016/j.jhazmat.2023.131752 Saeed Ur Rahman 1 , Muhammad Khalid 2 , Nan Hui 3 , Asad Rehman 3 , Sadaf-Ilyas Kayani 4 , Xueqing Fu 1 , Han Zheng 1 , Jin Shao 1 , Abid Ali Khan 5 , Mehran Ali 3 , Ayat Taheri 1 , Hang Liu 1 , Xin Yan 1 , Xinyi Hu 1 , Wei Qin 1 , Bowen Peng 1 , Meng Li 1 , Yao Xinghao 1 , Yaojie Zhang 1 , Kexuan Tang 1
Microorganisms in the rhizosphere are crucial allies for plant stress tolerance. Recent research suggests that by interacting with the rhizosphere microbiome, microorganisms can aid in the revegetation of soils contaminated with heavy metal(loid)s (HMs). However, it is unknown that how Piriformospora indica influences the rhizosphere microbiome to mitigate arsenic-toxicity in arsenic-enriched environments. Artemisia annua plants were grown in the presence or absence of P. indica and spiked with low (50) and high (150 µmol/L) concentrations of arsenic (As). After inoculation with P. indica, fresh weight increased by 37.7% and 10% in control and high concentration treated plants, respectively. Transmission electron microscopy showed that cellular organelles were severely damaged by As and even disappeared under high concentration. Furthermore, As was mostly accumulated by 5.9 and 18.1 mg/kg dry weight in the roots of inoculated plants treated with low and high concentrations of As, respectively. Additionally, 16 S and ITS rRNA gene sequencing were applied to analyze the rhizosphere microbial community structure of A. annua under different treatments. A significant difference was observed in microbial community structure under different treatments as revealed by non-metric multidimensional scaling ordination. The bacterial and fungal richness and diversity in the rhizosphere of inoculated plants were actively balanced and regulated by P. indica co-cultivation. Lysobacter and Steroidobacter were found to be the As-resistant bacterial genera. We conclude that P. indica inoculation could alter rhizosphere microecology, thereby mitigating As-toxicity without harming the environment.
中文翻译:
印度梨形孢菌改变根部相关微生物组结构以增强青蒿对砷的耐受性
根际微生物是植物抗逆性的重要盟友。最近的研究表明,通过与根际微生物组相互作用,微生物可以帮助被重金属(HMs)污染的土壤重新植被。然而,目前尚不清楚印度梨形孢菌如何影响根际微生物组以减轻富砷环境中的砷毒性。青蒿植物在存在或不存在青蒿的情况下生长。indica并掺入低浓度 (50 µmol/L) 和高浓度 (150 µmol/L) 砷 (As)。接种P后。籼稻,对照植物和高浓度处理植物的鲜重分别增加了 37.7% 和 10%。透射电镜观察表明,高浓度砷对细胞器的损伤严重,甚至消失。此外,在用低浓度和高浓度砷处理的接种植物的根部中,砷主要积累了5.9和18.1毫克/千克干重。此外,还应用 16 S 和 ITS rRNA 基因测序分析了A的根际微生物群落结构。青蒿在不同的治疗下。非度量多维尺度排序揭示了不同处理下微生物群落结构的显着差异。接种植物根际的细菌和真菌丰富度和多样性受到P的主动平衡和调节。籼稻共耕。溶杆菌属和类固醇杆菌属被发现是抗砷细菌属。我们的结论是P。籼稻接种可以改变根际微生态,从而在不损害环境的情况下减轻砷毒性。