Cadmium (Cd), a widespread and highly toxic environmental contaminant, has seriously impacted the growth of rice and the quality of its products. Hence, it is crucial to monitor and employ robust means to reduce Cd levels in rice, and selenium (Se) has been proven to chelate cadmium ion (Cd²⁺) in rice with rational use. Herein, for the first time, the reported selenocysteine (Sec) probe NN-Sec and the newly designed Cd²⁺ probe SCP were chosen as visualization tools to monitor Sec-inhibited Cd²⁺ uptake in rice. Specifically, reduced fluorescence of rice precultured with Cd²⁺ was observed by SCP after Se application, while similarly decreased fluorescence of rice pretreated with Se was observed by NN-Sec after Cd²⁺ addition. The diminished fluorescence indicated the formation of Cd-Se complexes reduced the Cd²⁺ content in rice. Additionally, it was Cd²⁺ and Se that entered the rice causing the fluorescence generation, as demonstrated by inductively coupled plasma mass spectrometry (ICP-MS). To conclude, the two probes successfully visualized Se inhibited Cd²⁺ uptake in rice, which could provide a robust tool for supporting the development of novel organic fertilizers and reagents to reduce Cd²⁺ content in rice and the environment.

镉（Cd）是一种广泛存在的高毒环境污染物，严重影响水稻的生长及其产品的质量。因此，监测和采用强有力的手段降低大米中的镉含量至关重要，硒（Se）已被证明可以螯合大米中的镉离子（Cd ²⁺），合理使用。在此，首次选择报道的硒代半胱氨酸（Sec）探针NN-Sec和新设计的Cd ²⁺探针SCP作为可视化工具来监测水稻中Sec抑制的Cd ^2+吸收。具体而言，通过SCP观察到用 Cd ²⁺预培养的水稻荧光减弱施用 Se 后，而在添加Cd ^{2+后，通过}NN-Sec观察到用 Se 预处理的水稻荧光同样降低。荧光减弱表明Cd-Se复合物的形成降低了水稻中Cd ^{2+的含量。}此外，电感耦合等离子体质谱 (ICP-MS) 证明，进入水稻的Cd ^{2+和 Se 导致荧光产生。}总之，这两个探针成功地观察到 Se 抑制水稻中 Cd ²⁺的吸收，这可以为支持新型有机肥料和试剂的开发以降低水稻和环境中的Cd ^{2+含量提供强大的工具。}