Translation of maternal mRNAs is detected before transcription of zygotic genes and is essential for mammalian embryo development. How certain maternal mRNAs are selected for translation instead of degradation and how this burst of translation affects zygotic genome activation remain unknown. Using gene-edited mice, we document that the oocyte-specific eukaryotic translation initiation factor 4E family member 1b (eIF4E1b) is the regulator of maternal mRNA expression that ensures subsequent reprogramming of the zygotic genome. In oocytes, eIF4E1b binds to transcripts encoding translation machinery proteins, chromatin remodelers, and reprogramming factors to promote their translation in zygotes and protect them from degradation. The protein products are thought to establish an open chromatin landscape in one-cell zygotes to enable transcription of genes required for cleavage stage development. Our results define a program for rapid resetting of the zygotic epigenome that is regulated by maternal mRNA expression and provide new insights into the mammalian maternal-to-zygotic transition.

中文翻译：

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生殖细胞特异性 eIF4E1b 调节母体 mRNA 翻译以确保合子基因组激活

母体 mRNA 的翻译在合子基因转录之前被检测到，并且对于哺乳动物胚胎发育至关重要。如何选择某些母体 mRNA 进行翻译而不是降解，以及这种翻译爆发如何影响合子基因组激活仍然未知。使用基因编辑小鼠，我们记录了卵母细胞特异性真核翻译起始因子 4E 家族成员 1b (eIF4E1b) 是母体 mRNA 表达的调节因子，可确保合子基因组的后续重编程。在卵母细胞中，eIF4E1b 与编码翻译机器蛋白、染色质重塑剂和重编程因子的转录物结合，以促进它们在受精卵中的翻译并保护它们免遭降解。这些蛋白质产品被认为在单细胞受精卵中建立了开放的染色质景观，以使裂解阶段发育所需的基因能够转录。我们的结果定义了一个快速重置受母体 mRNA 表达调节的合子表观基因组的程序，并为哺乳动物母体到合子的转变提供了新的见解。