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Forskolin improves salt tolerance of Gossypium hirsutum L. by upregulation of GhLTI65
Industrial Crops and Products ( IF 5.9 ) Pub Date : 2023-05-30 , DOI: 10.1016/j.indcrop.2023.116900
Peilin Wang, Xin Nie, Yulu Ye, Muhammad Ali Abid, Chengzhen Liang, Rui Zhang, Yuan Wang, Yunxiao Wei, Jiankui Zhang, Zhigang Meng

Cyclic AMP (cAMP) is a pivotal signaling molecule existing in almost all living organisms. It is an intracellular non-protein small molecule that regulates the activity of intracellular enzymes and non-enzyme proteins when its concentration changes in response to the binding of extracellular signals to cell surface receptors. As such, cAMP is referred to as a second messenger with an important role in cell signaling and modulation a variety of cellular responses. cAMP can help plants cope with abiotic stress, but the mechanism by which it improves salt tolerance has not been elucidated. In our study, we treated cotton with Forskolin (a cAMP activator) to increase cAMP levels, and found that it improved cotton seed tolerance to salt stress, thereby improving the germination rate of seeds in saline soil. Through transcriptome analysis, we characterized the responses of ABA, proteins, and pathways to salt stress after cAMP signaling, and four genes related to salt tolerance and signal transduction were identified. Three of them, GhLTI65, GhGOLS2, and GhNAC002 were silenced by virus induced gene silencing (VIGs), and the plant tolerance to salt stress was significantly reduced as compared with the control plants. Among these, we found that GhLTI65 (RD29B) was not only involved in the salt stress response, but played a role in the cAMP signaling cascade. We further performed yeast two-hybrid assays and bimolecular fluorescence complementation (BiFC) to determine that GhLTI65 interacts with the SNF1-related protein kinase GhPV42A in the cytoplasm, and that their co-expression is regulated by ABA-related signaling. We propose that ABA and cAMP signaling increase the expression of GhPV42A by integrating related mitogen-activated protein kinase (MAPK)cascade signaling, and that GhPV42A further interacts with GhLTI65 to increase its expression, thereby helping plants to receive signals and to respond to salt stress.



中文翻译:

Forskolin 通过上调 GhLTI65 提高陆地棉的耐盐性

环腺苷酸 (cAMP) 是几乎所有生物体中都存在的关键信号分子。它是一种细胞内非蛋白质小分子,当其浓度发生变化以响应细胞外信号与细胞表面受体的结合时,可调节细胞内酶和非酶蛋白的活性。因此,cAMP 被称为第二信使,在细胞信号传导和调节各种细胞反应中起着重要作用。cAMP 可以帮助植物应对非生物胁迫,但其提高耐盐性的机制尚未阐明。在我们的研究中,我们用 Forskolin(一种 cAMP 激活剂)处理棉花以提高 cAMP 水平,并发现它提高了棉花种子对盐胁迫的耐受性,从而提高了盐渍土中种子的发芽率。通过转录组分析,我们对 cAMP 信号后 ABA、蛋白质和盐胁迫通路的反应进行了表征,并鉴定了四个与耐盐性和信号转导相关的基因。其中三个,GhLTI65GhGOLS2GhNAC002被病毒诱导基因沉默 (VIG) 沉默,与对照植物相比,植物对盐胁迫的耐受性显着降低。其中,我们发现GhLTI65 ( RD29B )不仅参与盐胁迫反应,还在cAMP信号级联中发挥作用。我们进一步进行了酵母双杂交测定和双分子荧光互补 (BiFC),以确定GhLTI65与细胞质中的SNF1 相关蛋白激酶GhPV42A相互作用,并且它们的共表达受 ABA 相关信号传导调节。我们建议 ABA 和 cAMP 信号增加表达GhPV42A通过整合相关的丝裂原活化蛋白激酶(MAPK)级联信号,GhPV42A进一步与GhLTI65相互作用,增加其表达,从而帮助植物接收信号,响应盐胁迫。

更新日期:2023-05-30
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