The morphology of pseudomycelium in Trichosporon fermentans is not conducive to the accumulation of lipids and the recovery of yeast cells by sedimentation. In this study, the computational fluid dynamics method was used to study the effects of shear force on the flow field distribution and on the dimorphism transition and dispersion of yeast cells in a 5 L stirred bioreactor, and to explore the sedimentation performance of yeast cells in different forms. It was shown that under the condition of moderate shear force, such as 3.45 Pa corresponding to a stirring speed of 300 rpm as selected in this experiment, most of the yeast cells in the fermentation broth were spherical or short rod-shaped, and there were almost no pseudohyphal yeasts, which contributed to the good sedimentation performance of the yeast cells. The results showed that the appropriate shear force could effectively inhibit the transformation of yeast cells into pseudohyphae, retain a large number of cells in a single cell shape, enhance cell dispersion and facilitate cell contact, absorption and utilization of organic substrates, as well as increase the lipid accumulation in yeast cells. By controlling the shear force, after 40 h of treatment of RSOW using Trichosporon fermentans, the chemical oxygen demand and oil content removal were approximately 95.81% and 88.09%, respectively, which indicate a significant improvement compared with those before optimizing the shear environment. Therefore, based on shear control of the cell morphology to improve yeast cell dispersion and sedimentation performance, the oily wastewater treatment method established in this study can be used to guide the process design of fermented Trichosporon fermentans for the treatment of RSOW and the resource recycling of yeasts.

发酵丝孢子菌假菌丝体的形态不利于脂质的积累和酵母细胞沉降的恢复。本研究采用计算流体力学方法，研究了剪切力对5 L搅拌生物反应器中流场分布和酵母细胞二态转变及分散的影响，探讨了酵母细胞在5 L搅拌生物反应器中的沉降性能。不同的形式。结果表明，在中等剪切力条件下，如本实验选择3.45 Pa对应搅拌速度300 rpm，发酵液中的酵母细胞大部分呈球形或短棒状，有几乎没有假菌丝酵母，这有助于酵母细胞的良好沉降性能。结果表明，合适的剪切力能有效抑制酵母细胞向假菌丝转化，大量细胞保持单细胞形态，增强细胞分散性，促进细胞接触、吸收和利用有机底物，增加酵母细胞中的脂质积累。通过控制剪切力，在使用 RSOW 处理 40 小时后Trichosporon fermentans、化学需氧量和含油量去除率分别约为95.81%和88.09%，与优化剪切环境前相比有显着改善。因此，本研究建立的含油废水处理方法基于细胞形态的剪切控制以改善酵母细胞分散和沉降性能，可用于指导发酵丝孢子菌处理RSOW的工艺设计和资源回收利用。酵母。