Fungi in the sooty blotch and flyspeck (SBFS) disease complex appear as pigmented blemishes on plant cuticles and are commonly observed on several fruits, including mango. In Malaysia, SBFS on mango has not been reported previously. However, SBFS symptoms were observed in commercial mango orchards in Perak, resulting in economic losses for sale of fresh fruit. Therefore, morphological, molecular, and pathogenicity studies were conducted to identify SBFS taxa on mango in Perak. A total of 27 mango fruit showing SBFS blemishes were collected from several commercial orchards in Perak. Colony morphologies were described on fruit and in purified culture, and DNA was extracted from the cultures. The internal transcribed spacer (ITS) and large subunit (LSU) regions of ribosomal DNA of two representative isolates were amplified, sequenced, and deposited in GenBank. Maximum parsimony (MP) and maximum likelihood (ML) methods were used to construct phylogenetic trees. A modified version of Koch's postulates was performed for the two isolates using unripe, asymptomatic fruit with two inoculation methods: in situ in an orchard and on excised fruit. In each case, two fruit per isolate were inoculated and two additional fruit were sprayed with distilled water as a control. For the in situ inoculation, an additional two non-inoculated fruit were enclosed in bags until harvest. The colonies resulting from the inoculation of mango fruit matched the original isolates in mycelial type and ITS sequence. Two previously unknown Peltaster-like species were identified as casual fungi of SBFS on mango in Malaysia. To increase management effectiveness, more extensive research on SBFS phylogenetic diversity in Malaysia should be conducted.

黑斑病和蝇斑病 (SBFS) 综合症中的真菌表现为植物表皮上的色素斑点，常见于多种水果，包括芒果。在马来西亚，以前没有报道过芒果的 SBFS。然而，在霹雳州的商业芒果园中观察到 SBFS 症状，导致新鲜水果销售的经济损失。因此，进行了形态学、分子学和致病性研究，以确定霹雳州芒果的 SBFS 分类群。从霹雳州的几个商业果园收集了总共 27 个显示 SBFS 瑕疵的芒果果实。在水果和纯化培养物中描述了菌落形态，并从培养物中提取了 DNA。内部转录间隔区两个代表性分离株的核糖体 DNA 的 (ITS) 和大亚基 (LSU) 区域被扩增、测序并存放在 GenBank 中。使用最大简约法 (MP) 和最大似然法 (ML) 构建系统发育树。使用未成熟、无症状的水果和两种接种方法对两种分离株进行了科赫假设的修改版本：果园原位和切下的水果。在每种情况下，每个分离株接种两个果实，另外两个果实喷洒蒸馏水作为对照。对于原位接种后，将另外两个未接种的水果装入袋中直至收获。接种芒果果实产生的菌落在菌丝体类型和 ITS 序列上与原始分离株相匹配。两种以前未知的Peltaster样物种被确定为马来西亚芒果上的 SBFS 偶然真菌。为了提高管理效率，应该对马来西亚的 SBFS 系统发育多样性进行更广泛的研究。