Increased production of biogas and volatile acids was evaluated by the addition of Fe⁰ Nanoparticles (NP) on Anaerobic Fluidized Bed Reactor (AFBR-NP) with expanded clay as support material (45.1 mg Fe⁰ g⁻¹ expanded clay), fermenting vinasse, and operated at 37 °C and 3h Hydraulic Retention Time (HRT). Higher H₂ percentage in the biogas composition was observed in AFBR-NP (50–65%) when compared to AFBR without NP, AFBR-Control (24–43%). A 1.2-fold higher valeric acid production was also observed as a result of NPs supplementation. Genes related to the first steps of vinasse anaerobic degradation in AFBR-NP (beta-glucosidase, glucokinase and pyruvate-ferredoxin oxidoreductase) and the genes related to volatile acids production (L- and d-lactate dehydrogenase, 3-hydroxybutyryl-CoA dehydrogenase, acetate kinase, pyruvate kinase and fumarate reductase flavoprotein) were observed by metagenome sequencing. A 4.2-fold higher relative abundance of Secundilactobacillus genus in AFBR-NP was evidenced. The genetic and microbial community differences observed with the addition of NP resulted in improved biorefinery products of anaerobic digestion.

通过在厌氧流化床反应器 (AFBR-NP) 上添加 Fe ⁰纳米颗粒 (NP) 以膨胀粘土作为支持材料（45.1 mg Fe ⁰ g ^-1膨胀粘土）、发酵酒糟、并在 37°C 和 3 小时水力保留时间 (HRT) 下操作。较高的H ₂与没有 NP 的 AFBR、AFBR-Control (24–43%) 相比，在 AFBR-NP (50–65%) 中观察到沼气成分的百分比。由于补充 NP，还观察到戊酸产量提高了 1.2 倍。与 AFBR-NP 中酒糟厌氧降解第一步相关的基因（β-葡萄糖苷酶、葡萄糖激酶和丙酮酸铁氧还蛋白氧化还原酶）和与挥发性酸产生相关的基因（L-和 d-乳酸脱氢酶、3-羟基丁酰辅酶 A脱氢酶、醋酸激酶、丙酮酸激酶和富马酸还原酶黄素蛋白）通过宏基因组测序观察。Secundilactobacillus的相对丰度高 4.2 倍证明了 AFBR-NP 中的属。添加 NP 后观察到的遗传和微生物群落差异导致厌氧消化的生物精炼产品得到改善。