Life molecules’ distributions in live systems construct the complex dynamic reaction networks, whereas it is still challenging to demonstrate the dynamic distributions of biomolecules in live systems. Herein, we proposed a dynamic analysis strategy via sequence-structure bispecific RNA with state-adjustable molecules to monitor the dynamic concentration and spatiotemporal localization of these biomolecules in live cells based on the new insight of fluorescent RNA (FLRNA) interactions and their mechanism of fluorescence enhancement. Typically, computer-based nucleic acid-molecular docking simulation and molecular theoretical calculation have been proposed to provide a simple and straightforward method for guiding the custom-design of FLRNA. Impressively, a novel FLRNA with sequence and structure bispecific RNA named as a structure-switching aptamer (SSA) was introduced to monitor the real-time concentration and spatiotemporal localization of biomolecules, contributing to a deeper insight of the dynamic monitoring and visualization of biomolecules in live systems.

中文翻译：

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通过序列结构双特异性荧光 RNA 洞察生命分子在活细胞中的动态分布

生命系统中生命分子的分布构成了复杂的动态反应网络，而证明生命系统中生物分子的动态分布仍然具有挑战性。在此，我们提出了一种动态分析策略，通过基于荧光 RNA (FLRNA) 相互作用及其荧光增强机制的新见解，具有状态可调分子的序列结构双特异性 RNA 可监测活细胞中这些生物分子的动态浓度和时空定位。通常，已经提出了基于计算机的核酸-分子对接模拟和分子理论计算，以提供一种简单直接的方法来指导 FLRNA 的定制设计。令人印象深刻的是，引入了一种名为结构转换适配体 (SSA) 的具有序列和结构双特异性 RNA 的新型 FLRNA 来监测生物分子的实时浓度和时空定位，有助于更深入地了解生物分子的动态监测和可视化。实时系统。