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A Mitochondrial-targeted curcumin-based fluorescent probe for real-time tracking endogenous hydrogen peroxide in living cells
Sensors and Actuators B: Chemical ( IF 8.4 ) Pub Date : 2023-05-29 , DOI: 10.1016/j.snb.2023.134039
Mingming Zhu, Ting Wang, Yutao Zhang, Jiahuan Nong, Zhiqian Guo

To explore high-performance fluorescent probes that endow tunability and capability for in vivo analysis is an enduring goal of visualizing and manipulating biological events. Hydrogen peroxide H2O2, as a central redox signaling molecule, is the tough hurdle to real-time in situ tracking dynamic subcellular H2O2. Herein, we present a curcuminoid difluoroboron based fluorescent probe DFB-1 which contains the triphenylphosphine (TPP) moiety as mitochondrial targeting unit and aryl boronates as the specific uncaging group. Notably, this activatable probe DFB-1 bestows several striking characteristics: (i) dual-channel fluorescence response with a large shift; (ii) active mitochondria-targeting; (iii) light-up NIR fluorescence with high signal-to-background ratio. The probe DFB-1 is successfully employed in sensing endogenous H2O2 with a limit of detection 0.025 μM, and effectively differentiate H2O2 fluctuation between hepatocellular carcinoma and colorectal cancer cells. This dual-channel NIR probe DFB-1 realizes new advances in deep insight into the function of mitochondrial endogenous H2O2.



中文翻译:

一种基于线粒体靶向姜黄素的荧光探针,用于实时跟踪活细胞中的内源性过氧化氢

探索赋予体内分析可调性和能力的高性能荧光探针是可视化和操纵生物事件的持久目标。过氧化氢 H 2 O 2作为中心氧化还原信号分子,是实时原位追踪动态亚细胞 H 2 O 2的难关。在此,我们提出了一种基于姜黄素二氟硼的荧光探针DFB-1,它包含三苯基膦 (TPP) 部分作为线粒体靶向单元,芳基硼酸盐作为特定的解笼基团。值得注意的是,这个可激活的探针DFB-1赋予了几个显着的特征:(i)具有大偏移的双通道荧光响应;(ii) 主动靶向线粒体;(iii) 点亮具有高信号背景比的 NIR 荧光。该探针DFB-1成功用于检测内源性H 2 O 2,​​检测限为0.025 μM,可有效区分肝癌细胞和结直肠癌细胞之间的H 2 O 2波动。这种双通道 NIR 探针DFB-1实现了深入了解线粒体内源性 H 2 O 2功能的新进展。

更新日期:2023-05-31
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