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Insights into the driving factors of vertical distribution of antibiotic resistance genes in long-term fertilized soils
Journal of Hazardous Materials ( IF 13.6 ) Pub Date : 2023-05-24 , DOI: 10.1016/j.jhazmat.2023.131706
Ying Li 1 , Fanguang Kong 1 , Si Li 2 , Jie Wang 1 , Jingrun Hu 3 , Shuo Chen 1 , Qing Chen 1 , Yanming Li 1 , Xuejiao Ha 4 , Weiling Sun 3
Affiliation  

The prevalence of antibiotic resistance genes (ARGs) in soils has aroused wide attention. However, the influence of long-term fertilization on the distribution of ARGs in different soil layers and its dominant drivers remain largely unknown. In this study, a total of 203 ARGs were analyzed in greenhouse vegetable soils (0–100 cm from a 13-year field experiment applied with different fertilizers (control, chemical fertilizer, organic manure, and mixed fertilizer). Compared with unfertilized and chemically fertilized soils, manure application significantly increased the abundance and alpha diversity of soil ARGs, where the assembly of ARG communities was strongly driven by stochastic processes. The distribution of ARGs was significantly driven by manure application within 60 cm, while it was insignificantly changed in soil below 60 cm under different fertilization regimes. The inter-correlations of ARGs with mobile genetic elements (MGEs) and microbiota were strengthened in manured soil, indicating manure application posed a higher risk for ARGs diffusion in subsurface soil. Bacteria abundance and MGEs directly influenced ARG abundance and composition, whereas soil depth and manure application indirectly influenced ARG abundance and composition by affecting antibiotics. These results strengthen our understanding of the long-term anthropogenic influence on the vertical distribution of soil ARGs and highlight the ecological risk of ARGs in subsurface soil induced by long-term manure application.



中文翻译:

长期施肥土壤中抗生素抗性基因垂直分布驱动因素的洞察

土壤中抗生素抗性基因(ARGs)的普遍存在引起了广泛关注。然而,长期施肥对不同土壤层中 ARGs 分布的影响及其主要驱动因素在很大程度上仍然未知。在这项研究中,总共分析了温室蔬菜土壤中的 203 种 ARGs(0-100 厘米,来自 13 年的田间试验,施用了不同的肥料(控制、化肥、有机肥料和混合肥料)。与未施肥和化学肥料相比在施肥的土壤中,施肥显着增加了土壤 ARGs 的丰度和 alpha 多样性,其中 ARG 群落的组装受随机过程的强烈驱动。ARGs 的分布在 60 cm 范围内显着受施肥驱动,而在不同施肥方式下,60 cm以下土壤变化不显着。施肥土壤中 ARGs 与移动遗传元件 (MGEs) 和微生物群的相互关系得到加强,表明施肥对 ARGs 在地下土壤中扩散的风险更高。细菌丰度和 MGEs 直接影响 ARG 丰度和组成,而土壤深度和施肥通过影响抗生素间接影响 ARG 丰度和组成。这些结果加强了我们对长期人为影响对土壤 ARGs 垂直分布的理解,并突出了长期施肥引起的地下土壤 ARGs 的生态风险。施肥土壤中 ARGs 与移动遗传元件 (MGEs) 和微生物群的相互关系得到加强,表明施肥对 ARGs 在地下土壤中扩散的风险更高。细菌丰度和 MGEs 直接影响 ARG 丰度和组成,而土壤深度和施肥通过影响抗生素间接影响 ARG 丰度和组成。这些结果加强了我们对长期人为影响对土壤 ARGs 垂直分布的理解,并突出了长期施肥引起的地下土壤 ARGs 的生态风险。施肥土壤中 ARGs 与移动遗传元件 (MGEs) 和微生物群的相互关系得到加强,表明施肥对 ARGs 在地下土壤中扩散的风险更高。细菌丰度和 MGEs 直接影响 ARG 丰度和组成,而土壤深度和施肥通过影响抗生素间接影响 ARG 丰度和组成。这些结果加强了我们对长期人为影响对土壤 ARGs 垂直分布的理解,并突出了长期施肥引起的地下土壤 ARGs 的生态风险。而土壤深度和施肥通过影响抗生素间接影响 ARG 的丰度和组成。这些结果加强了我们对长期人为影响对土壤 ARGs 垂直分布的理解,并突出了长期施肥引起的地下土壤 ARGs 的生态风险。而土壤深度和施肥通过影响抗生素间接影响 ARG 的丰度和组成。这些结果加强了我们对长期人为影响对土壤 ARGs 垂直分布的理解,并突出了长期施肥引起的地下土壤 ARGs 的生态风险。

更新日期:2023-05-27
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