VIGS (Virus-induced gene silencing), a method for posttranscriptional gene silencing, is an effective technique for investigating the activities of genes in plants. Since there is no report for available VIGS system in Styrax japonicus, the application of a VIGS approach that results in a gene knockdown to study gene function is limited. In this study, we compared the characteristics that could affect the viability of VIGS in S. japonicus, including the acetosyringone (AS) concentration, the Agrobacterium's optical density and the inoculation method. The stable reference genes of S. japonicus were selected to validate the gene's knockdown by quantitative PCR. As a result, we successfully constructed 2 VIGS systems based on TRV virus: vacuum with AS concentration of 200 μmol·L⁻¹ and OD₆₀₀ of 0.5, and friction-osmosis with AS concentration of 200 μmol·L⁻¹ and OD₆₀₀ of 1.0, which silencing efficiency was 83.33% and 74.19%, respectively. The successfully applied VIGS method provides a rapid and effective reverse gene functional analysis approach in S. japonicus to identify unknown gene functions.

VIGS（病毒诱导基因沉默）是一种转录后基因沉默方法，是研究植物基因活性的有效技术。由于在安息香中没有可用的 VIGS 系统的报道，导致基因敲低的 VIGS 方法在研究基因功能中的应用受到限制。在本研究中，我们比较了影响日本血吸虫VIGS 活力的特性，包括乙酰丁香酮 (AS) 浓度、农杆菌光密度和接种方法。选择日本血吸虫稳定的内参基因，通过定量PCR验证该基因的敲低。结果，我们成功构建了2个基于TRV病毒的VIGS系统：AS浓度为200 μmol·L ^-1、OD _{600为0.5的真空系统，以及AS浓度为200 μmol·L} ^-1、OD ₆₀₀为0.5的摩擦渗透系统。1.0，沉默效率分别为83.33%和74.19%。VIGS方法的成功应用为刺参中未知基因功能的鉴定提供了一种快速有效的反向基因功能分析方法。