Foliar application of essential nutrients is a rapid and promising strategy to enhance the concentration and bioavailability of essential nutrients in tea plants (Camellia sinensis). A field experiment was carried out to explore the effects of iron (Fe), zinc (Zn), copper (Cu) and the hyperaccumulator manganese (Mn) foliar application on the biochemical components of free amino acid (FAA), caffeine (CAF), and tea polyphenols (TP) in the black tea made from the tea plant cultivar ‘HuangDan’. The foliar application of Fe, Zn, Cu, and Mn showed significant effects on FAA and TP contents of black tea (P < 0.05), but had no significant effect on the CAF content. Iron and zinc spraying significantly increased thearubigin and theabrownin content, whereas copper spraying significantly decreased thearubigin content while increasing theabrownin content. Additionally, the gene expression patterns of the POD family members CsSPX1, CsAPX1, CsGPX1, CsGPX3, CsPOD13, and CsPOD18 were identified to investigate the roles of these phenolic constituents influenced by the foliar application. The expression patterns of CsGPX3 and CsAPX1 were found to be involved in the production of TFs. POD-catalyzed TFs formation might serve as a molecular marker to identify varieties of tea plants suitable for brewing high-quality black tea beverages by regulating catechin oxidation throughout the tea processing process.

叶面喷施必需营养素是提高茶树 (Camellia sinensis) 必需营养素浓度和生物利用度的一种快速且有前途的策略。【摘要】：通过田间试验,探讨叶面喷施铁(Fe)、锌(Zn)、铜(Cu)和超积累植物锰(Mn)对游离氨基酸(FAA)、咖啡因(CAF)生化成分的影响。和茶树品种“黄丹”制成的红茶中的茶多酚 (TP)。叶面喷施Fe、Zn、Cu和Mn对红茶FAA和TP含量有显着影响（P < 0.05），但对CAF含量无显着影响。铁和锌喷洒显着增加了茶红素和茶褐素的含量，而喷铜显着降低了茶红素的含量，同时增加了茶褐素的含量。此外，还鉴定了 POD 家族成员 CsSPX1、CsAPX1、CsGPX1、CsGPX3、CsPOD13 和 CsPOD18 的基因表达模式，以研究这些酚类成分受叶面施用影响的作用。发现 CsGPX3 和 CsAPX1 的表达模式与 TF 的产生有关。POD 催化的 TF 形成可以作为分子标记，通过在整个茶叶加工过程中调节儿茶素氧化来鉴定适合酿造优质红茶饮料的茶树品种。发现 CsGPX3 和 CsAPX1 的表达模式与 TF 的产生有关。POD 催化的 TF 形成可以作为分子标记，通过在整个茶叶加工过程中调节儿茶素氧化来鉴定适合酿造高品质红茶饮料的茶树品种。发现 CsGPX3 和 CsAPX1 的表达模式与 TF 的产生有关。POD 催化的 TF 形成可以作为分子标记，通过在整个茶叶加工过程中调节儿茶素氧化来鉴定适合酿造优质红茶饮料的茶树品种。