While post-transcriptional control is thought to be required at the periphery of neurons and glia, its extent is unclear. Here, we investigate systematically the spatial distribution and expression of mRNA at single molecule sensitivity and their corresponding proteins of 200 YFP trap lines across the intact Drosophila nervous system. 97.5% of the genes studied showed discordance between the distribution of mRNA and the proteins they encode in at least one region of the nervous system. These data suggest that post-transcriptional regulation is very common, helping to explain the complexity of the nervous system. We also discovered that 68.5% of these genes have transcripts present at the periphery of neurons, with 9.5% at the glial periphery. Peripheral transcripts include many potential new regulators of neurons, glia, and their interactions. Our approach is applicable to most genes and tissues and includes powerful novel data annotation and visualization tools for post-transcriptional regulation.

中文翻译：

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YFP 陷阱的系统分析揭示了神经组织中常见的 mRNA/蛋白质不一致

虽然认为神经元和神经胶质细胞的外围需要转录后控制，但其程度尚不清楚。在这里，我们系统地研究了完整果蝇神经系统中 200 个 YFP 捕获线的 mRNA 在单分子敏感性下的空间分布和表达及其相应的蛋白质。97.5% 的研究基因显示，在神经系统的至少一个区域中，mRNA 与其编码的蛋白质的分布不一致。这些数据表明转录后调节非常常见，有助于解释神经系统的复杂性。我们还发现，这些基因中 68.5% 的转录本存在于神经元周围，其中 9.5% 存在于神经胶质周围。外周转录物包括神经元、神经胶质细胞及其相互作用的许多潜在的新调节因子。我们的方法适用于大多数基因和组织，并包括用于转录后调控的强大的新颖数据注释和可视化工具。